| http://purl.uniprot.org/citations/16762038 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16762038 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16762038 | http://www.w3.org/2000/01/rdf-schema#comment | "The gram-positive soil bacterium Cellulomonas fimi is shown to produce at least two intracellular beta-N-acetylglucosaminidases, a family 20 beta-N-acetylhexosaminidase (Hex20), and a novel family 3-beta-N-acetylglucosaminidase/beta-glucosidase (Nag3), through screening of a genomic expression library, cloning of genes and analysis of their sequences. Nag3 exhibits broad substrate specificity for substituents at the C2 position of the glycone: kcat/Km values at 25 degrees C were 0.066 s(-1) x mM(-1) and 0.076 s(-1) x mM(-1) for 4'-nitrophenyl beta-N-acetyl-D-glucosaminide and 4'-nitrophenyl beta-D-glucoside, respectively. The first glycosidase with this broad specificity to be described, Nag3, suggests an interesting evolutionary link between beta-N-acetylglucosaminidases and beta-glucosidases of family 3. Reaction by a double-displacement mechanism was confirmed for Nag3 through the identification of a glycosyl-enzyme species trapped with the slow substrate 2',4'-dinitrophenyl 2-deoxy-2-fluoro-beta-D-glucopyranoside. Hex20 requires the acetamido group at C2 of the substrate, being unable to cleave beta-glucosides, since its mechanism involves an oxazolinium ion intermediate. However, it is broad in its specificity for the D-glucosyl/D-galactosyl configuration of the glycone: Km and kcat values were 53 microM and 482.3 s(-1) for 4'-nitrophenyl beta-N-acetyl-D-glucosaminide and 66 microM and 129.1 s(-1) for 4'-nitrophenyl beta-N-acetyl-D-galactosaminide."xsd:string |
| http://purl.uniprot.org/citations/16762038 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1742-4658.2006.05308.x"xsd:string |
| http://purl.uniprot.org/citations/16762038 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1742-4658.2006.05308.x"xsd:string |
| http://purl.uniprot.org/citations/16762038 | http://purl.uniprot.org/core/author | "Mayer C."xsd:string |
| http://purl.uniprot.org/citations/16762038 | http://purl.uniprot.org/core/author | "Mayer C."xsd:string |
| http://purl.uniprot.org/citations/16762038 | http://purl.uniprot.org/core/author | "Withers S.G."xsd:string |
| http://purl.uniprot.org/citations/16762038 | http://purl.uniprot.org/core/author | "Withers S.G."xsd:string |
| http://purl.uniprot.org/citations/16762038 | http://purl.uniprot.org/core/author | "Vocadlo D.J."xsd:string |
| http://purl.uniprot.org/citations/16762038 | http://purl.uniprot.org/core/author | "Vocadlo D.J."xsd:string |
| http://purl.uniprot.org/citations/16762038 | http://purl.uniprot.org/core/author | "Rupitz K."xsd:string |
| http://purl.uniprot.org/citations/16762038 | http://purl.uniprot.org/core/author | "Rupitz K."xsd:string |
| http://purl.uniprot.org/citations/16762038 | http://purl.uniprot.org/core/author | "Warren R.A.J."xsd:string |
| http://purl.uniprot.org/citations/16762038 | http://purl.uniprot.org/core/author | "Warren R.A.J."xsd:string |
| http://purl.uniprot.org/citations/16762038 | http://purl.uniprot.org/core/author | "Stoll D."xsd:string |
| http://purl.uniprot.org/citations/16762038 | http://purl.uniprot.org/core/author | "Stoll D."xsd:string |
| http://purl.uniprot.org/citations/16762038 | http://purl.uniprot.org/core/author | "Mah M."xsd:string |
| http://purl.uniprot.org/citations/16762038 | http://purl.uniprot.org/core/author | "Mah M."xsd:string |
| http://purl.uniprot.org/citations/16762038 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16762038 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16762038 | http://purl.uniprot.org/core/name | "FEBS J."xsd:string |
| http://purl.uniprot.org/citations/16762038 | http://purl.uniprot.org/core/name | "FEBS J."xsd:string |
| http://purl.uniprot.org/citations/16762038 | http://purl.uniprot.org/core/pages | "2929-2941"xsd:string |
| http://purl.uniprot.org/citations/16762038 | http://purl.uniprot.org/core/pages | "2929-2941"xsd:string |