| http://purl.uniprot.org/citations/16775010 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16775010 | http://www.w3.org/2000/01/rdf-schema#comment | "Overexpression of the inhibitory Smad, Smad7, is used frequently to implicate the Smad pathway in cellular responses to transforming growth factor beta (TGF-beta) signaling; however, Smad7 regulates several other proteins, including Cdc42, p38MAPK, and beta-catenin. We report an alternative approach for more specifically disrupting Smad-dependent signaling using a peptide aptamer, Trx-SARA, which comprises a rigid scaffold, the Escherichia coli thioredoxin A protein (Trx), displaying a constrained 56-amino acid Smad-binding motif from the Smad anchor for receptor activation (SARA) protein. Trx-SARA bound specifically to Smad2 and Smad3 and inhibited both TGF-beta-induced reporter gene expression and epithelial-to-mesenchymal transition in NMuMG murine mammary epithelial cells. In contrast to Smad7, Trx-SARA had no effect on the Smad2 or 3 phosphorylation levels induced by TGF-beta1. Trx-SARA was primarily localized to the nucleus and perturbed the normal cytoplasmic localization of Smad2 and 3 to a nuclear localization in the absence of TGF-beta1, consistent with reduced Smad nuclear export. The key mode of action of Trx-SARA was to reduce the level of Smad2 and Smad3 in complex with Smad4 after TGF-beta1 stimulation, a mechanism of action consistent with the preferential binding of SARA to monomeric Smad protein and Trx-SARA-mediated disruption of active Smad complexes."xsd:string |
| http://purl.uniprot.org/citations/16775010 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.e05-10-0990"xsd:string |
| http://purl.uniprot.org/citations/16775010 | http://purl.uniprot.org/core/author | "Hoffmann F.M."xsd:string |
| http://purl.uniprot.org/citations/16775010 | http://purl.uniprot.org/core/author | "Zhao B.M."xsd:string |
| http://purl.uniprot.org/citations/16775010 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16775010 | http://purl.uniprot.org/core/name | "Mol Biol Cell"xsd:string |
| http://purl.uniprot.org/citations/16775010 | http://purl.uniprot.org/core/pages | "3819-3831"xsd:string |
| http://purl.uniprot.org/citations/16775010 | http://purl.uniprot.org/core/title | "Inhibition of transforming growth factor-beta1-induced signaling and epithelial-to-mesenchymal transition by the Smad-binding peptide aptamer Trx-SARA."xsd:string |
| http://purl.uniprot.org/citations/16775010 | http://purl.uniprot.org/core/volume | "17"xsd:string |
| http://purl.uniprot.org/citations/16775010 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16775010 |
| http://purl.uniprot.org/citations/16775010 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16775010 |
| http://purl.uniprot.org/uniprot/#_B3KXA7-mappedCitation-16775010 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16775010 |
| http://purl.uniprot.org/uniprot/#_Q7Z3T8-mappedCitation-16775010 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16775010 |
| http://purl.uniprot.org/uniprot/Q7Z3T8 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/16775010 |
| http://purl.uniprot.org/uniprot/B3KXA7 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/16775010 |