| http://purl.uniprot.org/citations/16784888 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16784888 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16784888 | http://www.w3.org/2000/01/rdf-schema#comment | "3Beta-hydroxysterol Delta(14)-reductase operates during the conversion of lanosterol to cholesterol in mammalian cells. Besides the endoplasmic reticulum 3beta-hydroxysterol Delta(14)-reductase (C14SR) encoded by TM7SF2 gene, the lamin B receptor (LBR) of the inner nuclear membrane possesses 3beta-hydroxysterol Delta(14)-reductase activity, based on its ability to complement C14SR-defective yeast strains. LBR was indicated as the primary 3beta-hydroxysterol Delta(14)-reductase in human cholesterol biosynthesis, since mutations in LBR gene were found in Greenberg skeletal dysplasia, characterized by accumulation of Delta(14)-unsaturated sterols. This study addresses the issue of C14SR and LBR role in cholesterol biosynthesis. Both human C14SR and LBR expressed in COS-1 cells exhibit 3beta-hydroxysterol Delta(14)-reductase activity in vitro. TM7SF2 mRNA and C14SR protein expression in HepG2 cells grown in delipidated serum (LPDS) plus lovastatin (sterol starvation) were 4- and 8-fold higher, respectively, than in LPDS plus 25-hydroxycholesterol (sterol feeding), resulting in 4-fold higher 3beta-hydroxysterol Delta(14)-reductase activity. No variations in LBR mRNA and protein levels were detected in the same conditions. The induction of TM7SF2 gene expression is turned-on by promoter activation in response to low cell sterol levels and is mediated by SREBP-2. The results suggest a primary role of C14SR in human cholesterol biosynthesis, whereas LBR role in the pathway remains unclear."xsd:string |
| http://purl.uniprot.org/citations/16784888 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbalip.2006.05.004"xsd:string |
| http://purl.uniprot.org/citations/16784888 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbalip.2006.05.004"xsd:string |
| http://purl.uniprot.org/citations/16784888 | http://purl.uniprot.org/core/author | "Roberti R."xsd:string |
| http://purl.uniprot.org/citations/16784888 | http://purl.uniprot.org/core/author | "Roberti R."xsd:string |
| http://purl.uniprot.org/citations/16784888 | http://purl.uniprot.org/core/author | "Castelli M."xsd:string |
| http://purl.uniprot.org/citations/16784888 | http://purl.uniprot.org/core/author | "Castelli M."xsd:string |
| http://purl.uniprot.org/citations/16784888 | http://purl.uniprot.org/core/author | "Della Fazia M.A."xsd:string |
| http://purl.uniprot.org/citations/16784888 | http://purl.uniprot.org/core/author | "Della Fazia M.A."xsd:string |
| http://purl.uniprot.org/citations/16784888 | http://purl.uniprot.org/core/author | "Servillo G."xsd:string |
| http://purl.uniprot.org/citations/16784888 | http://purl.uniprot.org/core/author | "Servillo G."xsd:string |
| http://purl.uniprot.org/citations/16784888 | http://purl.uniprot.org/core/author | "Caruso D."xsd:string |
| http://purl.uniprot.org/citations/16784888 | http://purl.uniprot.org/core/author | "Caruso D."xsd:string |
| http://purl.uniprot.org/citations/16784888 | http://purl.uniprot.org/core/author | "Beccari T."xsd:string |
| http://purl.uniprot.org/citations/16784888 | http://purl.uniprot.org/core/author | "Beccari T."xsd:string |
| http://purl.uniprot.org/citations/16784888 | http://purl.uniprot.org/core/author | "Bennati A.M."xsd:string |
| http://purl.uniprot.org/citations/16784888 | http://purl.uniprot.org/core/author | "Bennati A.M."xsd:string |
| http://purl.uniprot.org/citations/16784888 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16784888 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16784888 | http://purl.uniprot.org/core/name | "Biochim. Biophys. Acta"xsd:string |
| http://purl.uniprot.org/citations/16784888 | http://purl.uniprot.org/core/name | "Biochim. Biophys. Acta"xsd:string |
| http://purl.uniprot.org/citations/16784888 | http://purl.uniprot.org/core/pages | "677-685"xsd:string |
| http://purl.uniprot.org/citations/16784888 | http://purl.uniprot.org/core/pages | "677-685"xsd:string |