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http://purl.uniprot.org/citations/16787535http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/16787535http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/16787535http://www.w3.org/2000/01/rdf-schema#comment"

Background

Integrins comprise a large family of alpha,beta heterodimeric, transmembrane cell adhesion receptors that mediate diverse essential biological functions. Higher vertebrates possess a single beta1 gene, and the beta1 subunit associates with a large number of alpha subunits to form the major class of extracellular matrix (ECM) receptors. Despite the fact that the zebrafish (Danio rerio) is a rapidly emerging model organism of choice for developmental biology and for models of human disease, little is currently known about beta1 integrin sequences and functions in this organism.

Results

Using RT-PCR, complete coding sequences of zebrafish beta1 paralogs were obtained from zebrafish embryos or adult tissues. The results show that zebrafish possess two beta1 paralogs (beta1-1 and beta1-2) that have a high degree of identity to other vertebrate beta1 subunits. In addition, a third, more divergent, beta1 paralog is present (beta1-3), which may have altered ligand-binding properties. Zebrafish also have other divergent beta1-like transcripts, which are C-terminally truncated forms lacking the transmembrane and cytoplasmic domains. Together with beta1-3 these truncated forms comprise a novel group of beta1 paralogs, all of which have a mutation in the ADMIDAS cation-binding site. Phylogenetic and genomic analyses indicate that the duplication that gave rise to beta1-1 and beta1-2 occurred after the divergence of the tetrapod and fish lineages, while a subsequent duplication of the ancestor of beta1-2 may have given rise to beta1-3 and an ancestral truncated paralog. A very recent tandem duplication of the truncated beta1 paralogs appears to have taken place. The different zebrafish beta1 paralogs have varied patterns of temporal expression during development. Beta1-1 and beta1-2 are ubiquitously expressed in adult tissues, whereas the other beta1 paralogs generally show more restricted patterns of expression.

Conclusion

Zebrafish have a large set of integrin beta1 paralogs. beta1-1 and beta1-2 may share the roles of the solitary beta1 subunit found in other vertebrates, whereas beta1-3 and the truncated beta1 paralogs may have acquired novel functions."xsd:string
http://purl.uniprot.org/citations/16787535http://purl.org/dc/terms/identifier"doi:10.1186/1471-2121-7-24"xsd:string
http://purl.uniprot.org/citations/16787535http://purl.org/dc/terms/identifier"doi:10.1186/1471-2121-7-24"xsd:string
http://purl.uniprot.org/citations/16787535http://purl.uniprot.org/core/author"Mould A.P."xsd:string
http://purl.uniprot.org/citations/16787535http://purl.uniprot.org/core/author"Mould A.P."xsd:string
http://purl.uniprot.org/citations/16787535http://purl.uniprot.org/core/author"Hurlstone A.F."xsd:string
http://purl.uniprot.org/citations/16787535http://purl.uniprot.org/core/author"Hurlstone A.F."xsd:string
http://purl.uniprot.org/citations/16787535http://purl.uniprot.org/core/author"Boot-Handford R.P."xsd:string
http://purl.uniprot.org/citations/16787535http://purl.uniprot.org/core/author"Boot-Handford R.P."xsd:string
http://purl.uniprot.org/citations/16787535http://purl.uniprot.org/core/author"Humphries M.J."xsd:string
http://purl.uniprot.org/citations/16787535http://purl.uniprot.org/core/author"Humphries M.J."xsd:string
http://purl.uniprot.org/citations/16787535http://purl.uniprot.org/core/author"Huxley-Jones J."xsd:string
http://purl.uniprot.org/citations/16787535http://purl.uniprot.org/core/author"Huxley-Jones J."xsd:string
http://purl.uniprot.org/citations/16787535http://purl.uniprot.org/core/author"Goonesinghe A.C."xsd:string
http://purl.uniprot.org/citations/16787535http://purl.uniprot.org/core/author"Goonesinghe A.C."xsd:string
http://purl.uniprot.org/citations/16787535http://purl.uniprot.org/core/author"McLeish J.A."xsd:string
http://purl.uniprot.org/citations/16787535http://purl.uniprot.org/core/author"McLeish J.A."xsd:string
http://purl.uniprot.org/citations/16787535http://purl.uniprot.org/core/date"2006"xsd:gYear
http://purl.uniprot.org/citations/16787535http://purl.uniprot.org/core/date"2006"xsd:gYear
http://purl.uniprot.org/citations/16787535http://purl.uniprot.org/core/name"BMC Cell Biol."xsd:string
http://purl.uniprot.org/citations/16787535http://purl.uniprot.org/core/name"BMC Cell Biol"xsd:string
http://purl.uniprot.org/citations/16787535http://purl.uniprot.org/core/pages"24"xsd:string
http://purl.uniprot.org/citations/16787535http://purl.uniprot.org/core/pages"24"xsd:string
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