| http://purl.uniprot.org/citations/16790491 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16790491 | http://www.w3.org/2000/01/rdf-schema#comment | "Clathrin adaptors are key factors in clathrin-coated vesicle formation, coupling clathrin to cargo and/or the lipid bilayer. A physically interacting network of three classes of adaptors participate in clathrin-mediated traffic between the trans-Golgi network (TGN) and endosomes: AP-1, Gga proteins, and epsin-like proteins. Here we investigate functional relationships within this network through transport assays and protein localization analysis in living yeast cells. We observed that epsin-like protein Ent3p preferentially localized with Gga2p, whereas Ent5p distributed equally between AP-1 and Gga2p. Ent3p was mislocalized in Gga-deficient but not in AP-1-deficient cells. In contrast, Ent5p retained localization in cells lacking either or both AP-1 and Gga proteins. The Ent proteins were dispensable for AP-1 or Gga localization. Synthetic genetic growth and alpha-factor maturation defects were observed when ent5Delta but not ent3Delta was introduced together with deletions of the GGA genes. In AP-1-deficient cells, ent3Delta and to a lesser extent ent5Delta caused minor alpha-factor maturation defects, but together resulted in a near-lethal phenotype. Deletions of ENT3 and ENT5 also displayed synthetic defects similar to, but less severe than, synthetic effects of AP-1 and Gga inactivation. These results differentiate Ent3p and Ent5p function in vivo, suggesting that Ent3p acts primarily with Gga proteins, whereas Ent5p acts with both AP-1 and Gga proteins but is more critical for AP-1-mediated transport. The data also support a model in which the Ent adaptors provide important accessory functions to AP-1 and Gga proteins in TGN/endosome traffic."xsd:string |
| http://purl.uniprot.org/citations/16790491 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.e06-05-0410"xsd:string |
| http://purl.uniprot.org/citations/16790491 | http://purl.uniprot.org/core/author | "Payne G.S."xsd:string |
| http://purl.uniprot.org/citations/16790491 | http://purl.uniprot.org/core/author | "Duncan M.C."xsd:string |
| http://purl.uniprot.org/citations/16790491 | http://purl.uniprot.org/core/author | "Costaguta G."xsd:string |
| http://purl.uniprot.org/citations/16790491 | http://purl.uniprot.org/core/author | "Fernandez G.E."xsd:string |
| http://purl.uniprot.org/citations/16790491 | http://purl.uniprot.org/core/author | "Huang G.H."xsd:string |
| http://purl.uniprot.org/citations/16790491 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16790491 | http://purl.uniprot.org/core/name | "Mol Biol Cell"xsd:string |
| http://purl.uniprot.org/citations/16790491 | http://purl.uniprot.org/core/pages | "3907-3920"xsd:string |
| http://purl.uniprot.org/citations/16790491 | http://purl.uniprot.org/core/title | "Distinct roles for TGN/endosome epsin-like adaptors Ent3p and Ent5p."xsd:string |
| http://purl.uniprot.org/citations/16790491 | http://purl.uniprot.org/core/volume | "17"xsd:string |
| http://purl.uniprot.org/citations/16790491 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16790491 |
| http://purl.uniprot.org/citations/16790491 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16790491 |
| http://purl.uniprot.org/uniprot/#_A0A8H8UN61-mappedCitation-16790491 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16790491 |
| http://purl.uniprot.org/uniprot/#_Q03769-mappedCitation-16790491 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16790491 |
| http://purl.uniprot.org/uniprot/#_Q06336-mappedCitation-16790491 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16790491 |
| http://purl.uniprot.org/uniprot/#_P47160-mappedCitation-16790491 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16790491 |
| http://purl.uniprot.org/uniprot/#_P36000-mappedCitation-16790491 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16790491 |
| http://purl.uniprot.org/uniprot/#_P38817-mappedCitation-16790491 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16790491 |
| http://purl.uniprot.org/uniprot/Q06336 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/16790491 |
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