| http://purl.uniprot.org/citations/16809541 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16809541 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/16809541 | http://www.w3.org/2000/01/rdf-schema#comment | "The formation of glutaminyl transfer RNA (Gln-tRNA(Gln)) differs among the three domains of life. Most bacteria employ an indirect pathway to produce Gln-tRNA(Gln) by a heterotrimeric glutamine amidotransferase CAB (GatCAB) that acts on the misacylated Glu-tRNA(Gln). Here, we describe a series of crystal structures of intact GatCAB from Staphylococcus aureus in the apo form and in the complexes with glutamine, asparagine, Mn2+, and adenosine triphosphate analog. Two identified catalytic centers for the glutaminase and transamidase reactions are markedly distant but connected by a hydrophilic ammonia channel 30 A in length. Further, we show that the first U-A base pair in the acceptor stem and the D loop of tRNA(Gln) serve as identity elements essential for discrimination by GatCAB and propose a complete model for the overall concerted reactions to synthesize Gln-tRNA(Gln)."xsd:string |
| http://purl.uniprot.org/citations/16809541 | http://purl.uniprot.org/core/name | "Science"xsd:string |
| http://purl.uniprot.org/citations/16809541 | http://purl.org/dc/terms/identifier | "doi:10.1126/science.1127156"xsd:string |
| http://purl.uniprot.org/citations/16809541 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16809541 |
| http://purl.uniprot.org/citations/16809541 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16809541 |
| http://purl.uniprot.org/citations/16809541 | http://purl.uniprot.org/core/author | "Nakamura A."xsd:string |
| http://purl.uniprot.org/citations/16809541 | http://purl.uniprot.org/core/author | "Sakai N."xsd:string |
| http://purl.uniprot.org/citations/16809541 | http://purl.uniprot.org/core/author | "Tanaka I."xsd:string |
| http://purl.uniprot.org/citations/16809541 | http://purl.uniprot.org/core/author | "Yao M."xsd:string |
| http://purl.uniprot.org/citations/16809541 | http://purl.uniprot.org/core/author | "Chimnaronk S."xsd:string |
| http://purl.uniprot.org/citations/16809541 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16809541 | http://purl.uniprot.org/core/pages | "1954-1958"xsd:string |
| http://purl.uniprot.org/citations/16809541 | http://purl.uniprot.org/core/title | "Ammonia channel couples glutaminase with transamidase reactions in GatCAB."xsd:string |
| http://purl.uniprot.org/citations/16809541 | http://purl.uniprot.org/core/volume | "312"xsd:string |
| http://purl.uniprot.org/citations/16809541 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16809541 |
| http://purl.uniprot.org/citations/16809541 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16809541 |
| http://purl.uniprot.org/uniprot/#_P68807-mappedCitation-16809541 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16809541 |
| http://purl.uniprot.org/uniprot/#_P63488-mappedCitation-16809541 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16809541 |
| http://purl.uniprot.org/uniprot/#_P64201-mappedCitation-16809541 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16809541 |
| http://purl.uniprot.org/enzyme/6.3.5.7 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/16809541 |
| http://purl.uniprot.org/uniprot/P63488 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/16809541 |
| http://purl.uniprot.org/uniprot/P68807 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/16809541 |
| http://purl.uniprot.org/uniprot/P64201 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/16809541 |