| http://purl.uniprot.org/citations/16810316 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16810316 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16810316 | http://www.w3.org/2000/01/rdf-schema#comment | "The structural maintenance of chromosomes (SMC) family of proteins has been implicated in the repair of DNA double-strand breaks (DSBs) by homologous recombination (HR). The SMC1/3 cohesin complex is thought to promote HR by maintaining the close proximity of sister chromatids at DSBs. The SMC5/6 complex is also required for DNA repair, but the mechanism by which it accomplishes this is unclear. Here, we show that RNAi-mediated knockdown of the SMC5/6 complex components in human cells increases the efficiency of gene targeting due to a specific requirement for hSMC5/6 in sister chromatid HR. Knockdown of the hSMC5/6 complex decreases sister chromatid HR, but does not reduce nonhomologous end-joining (NHEJ) or intra-chromatid, homologue, or extrachromosomal HR. The hSMC5/6 complex is itself recruited to nuclease-induced DSBs and is required for the recruitment of cohesin to DSBs. Our results establish a mechanism by which the hSMC5/6 complex promotes DNA repair and suggest a novel strategy to improve the efficiency of gene targeting in mammalian somatic cells."xsd:string |
| http://purl.uniprot.org/citations/16810316 | http://purl.org/dc/terms/identifier | "doi:10.1038/sj.emboj.7601218"xsd:string |
| http://purl.uniprot.org/citations/16810316 | http://purl.org/dc/terms/identifier | "doi:10.1038/sj.emboj.7601218"xsd:string |
| http://purl.uniprot.org/citations/16810316 | http://purl.uniprot.org/core/author | "Yu H."xsd:string |
| http://purl.uniprot.org/citations/16810316 | http://purl.uniprot.org/core/author | "Yu H."xsd:string |
| http://purl.uniprot.org/citations/16810316 | http://purl.uniprot.org/core/author | "Potts P.R."xsd:string |
| http://purl.uniprot.org/citations/16810316 | http://purl.uniprot.org/core/author | "Potts P.R."xsd:string |
| http://purl.uniprot.org/citations/16810316 | http://purl.uniprot.org/core/author | "Porteus M.H."xsd:string |
| http://purl.uniprot.org/citations/16810316 | http://purl.uniprot.org/core/author | "Porteus M.H."xsd:string |
| http://purl.uniprot.org/citations/16810316 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16810316 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16810316 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/16810316 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/16810316 | http://purl.uniprot.org/core/pages | "3377-3388"xsd:string |
| http://purl.uniprot.org/citations/16810316 | http://purl.uniprot.org/core/pages | "3377-3388"xsd:string |
| http://purl.uniprot.org/citations/16810316 | http://purl.uniprot.org/core/title | "Human SMC5/6 complex promotes sister chromatid homologous recombination by recruiting the SMC1/3 cohesin complex to double-strand breaks."xsd:string |
| http://purl.uniprot.org/citations/16810316 | http://purl.uniprot.org/core/title | "Human SMC5/6 complex promotes sister chromatid homologous recombination by recruiting the SMC1/3 cohesin complex to double-strand breaks."xsd:string |
| http://purl.uniprot.org/citations/16810316 | http://purl.uniprot.org/core/volume | "25"xsd:string |
| http://purl.uniprot.org/citations/16810316 | http://purl.uniprot.org/core/volume | "25"xsd:string |
| http://purl.uniprot.org/citations/16810316 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16810316 |
| http://purl.uniprot.org/citations/16810316 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16810316 |
| http://purl.uniprot.org/citations/16810316 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16810316 |
| http://purl.uniprot.org/citations/16810316 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16810316 |