| http://purl.uniprot.org/citations/16858395 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16858395 | http://www.w3.org/2000/01/rdf-schema#comment | "B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK). B-Raf differs from the other Raf isoforms in that it has a long amino-terminal region. By the use of probes based on the principle of fluorescence resonance energy transfer, we found that this amino-terminal B-Raf-specific region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. HeLa cells expressing B-Raf, but not c-Raf, or a B-Raf mutant lacking the B-Raf-specific region, showed enhanced MEK phosphorylation upon stimulation with a calcium agonist. Furthermore, increases in the intracellular calcium concentration were found to be necessary for dimerization and sufficient for the plasma membrane translocation of B-Raf. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Thus, we propose that the hitherto unidentified function of the B-Raf amino-terminal region is to mediate calcium-dependent activation of B-Raf and the following MEK activation, which may occur in the absence of Ras activation."xsd:string |
| http://purl.uniprot.org/citations/16858395 | http://purl.org/dc/terms/identifier | "doi:10.1038/sj.emboj.7601241"xsd:string |
| http://purl.uniprot.org/citations/16858395 | http://purl.uniprot.org/core/author | "Matsuda M."xsd:string |
| http://purl.uniprot.org/citations/16858395 | http://purl.uniprot.org/core/author | "Terai K."xsd:string |
| http://purl.uniprot.org/citations/16858395 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16858395 | http://purl.uniprot.org/core/name | "EMBO J"xsd:string |
| http://purl.uniprot.org/citations/16858395 | http://purl.uniprot.org/core/pages | "3556-3564"xsd:string |
| http://purl.uniprot.org/citations/16858395 | http://purl.uniprot.org/core/title | "The amino-terminal B-Raf-specific region mediates calcium-dependent homo- and hetero-dimerization of Raf."xsd:string |
| http://purl.uniprot.org/citations/16858395 | http://purl.uniprot.org/core/volume | "25"xsd:string |
| http://purl.uniprot.org/citations/16858395 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16858395 |
| http://purl.uniprot.org/citations/16858395 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16858395 |
| http://purl.uniprot.org/uniprot/P15056#attribution-F27BEEBCEC11545F72316A2C7F4C86E2 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/16858395 |
| http://purl.uniprot.org/uniprot/#_P15056-mappedCitation-16858395 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16858395 |
| http://purl.uniprot.org/uniprot/P15056 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/16858395 |