| http://purl.uniprot.org/citations/16861810 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16861810 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16861810 | http://www.w3.org/2000/01/rdf-schema#comment | "A culture filtrate of Bacillus circulans KA-304 grown on a cell-wall preparation of Schizophyllum commune has an activity to form protoplasts from S. commune mycelia, and a combination of alpha-1,3-glucanase and chitinase I, which were isolated from the filtrate, brings about the protoplast-forming activity. The gene of alpha-1,3-glucanase was cloned from B. circulans KA-304. It consists of 3,879 nucleotides, which encodes 1,293 amino acids including a putative signal peptide (31 amino acid residues), and the molecular weight of alpha-1,3-glucanase without the putative signal peptide was calculated to be 132,184. The deduced amino acid sequence of alpha-1,3-glucanase of B. circulans KA-304 showed approximately 80% similarity to that of mutanase (alpha-1,3-glucanase) of Bacillus sp. RM1, but no significant similarity to those of fungal mutanases. The recombinant alpha-1,3-glucanase was expressed in Escherichia coli Rosetta-gami B (DE 3), and significant alpha-1,3-glucanase activity was detected in the cell-free extract of the organism treated with isopropyl-beta-D-thiogalactopyranoside. The recombinant alpha-1,3-glucanase showed protoplast-forming activity when the enzyme was combined with chitinase I."xsd:string |
| http://purl.uniprot.org/citations/16861810 | http://purl.org/dc/terms/identifier | "doi:10.1271/bbb.60095"xsd:string |
| http://purl.uniprot.org/citations/16861810 | http://purl.org/dc/terms/identifier | "doi:10.1271/bbb.60095"xsd:string |
| http://purl.uniprot.org/citations/16861810 | http://purl.uniprot.org/core/author | "Yano S."xsd:string |
| http://purl.uniprot.org/citations/16861810 | http://purl.uniprot.org/core/author | "Yano S."xsd:string |
| http://purl.uniprot.org/citations/16861810 | http://purl.uniprot.org/core/author | "Tachiki T."xsd:string |
| http://purl.uniprot.org/citations/16861810 | http://purl.uniprot.org/core/author | "Tachiki T."xsd:string |
| http://purl.uniprot.org/citations/16861810 | http://purl.uniprot.org/core/author | "Wakayama M."xsd:string |
| http://purl.uniprot.org/citations/16861810 | http://purl.uniprot.org/core/author | "Wakayama M."xsd:string |
| http://purl.uniprot.org/citations/16861810 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16861810 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16861810 | http://purl.uniprot.org/core/name | "Biosci. Biotechnol. Biochem."xsd:string |
| http://purl.uniprot.org/citations/16861810 | http://purl.uniprot.org/core/name | "Biosci Biotechnol Biochem"xsd:string |
| http://purl.uniprot.org/citations/16861810 | http://purl.uniprot.org/core/pages | "1754-1763"xsd:string |
| http://purl.uniprot.org/citations/16861810 | http://purl.uniprot.org/core/pages | "1754-1763"xsd:string |
| http://purl.uniprot.org/citations/16861810 | http://purl.uniprot.org/core/title | "Cloning and expression of an alpha-1,3-glucanase gene from Bacillus circulans KA-304: the enzyme participates in protoplast formation of Schizophyllum commune."xsd:string |
| http://purl.uniprot.org/citations/16861810 | http://purl.uniprot.org/core/title | "Cloning and expression of an alpha-1,3-glucanase gene from Bacillus circulans KA-304: the enzyme participates in protoplast formation of Schizophyllum commune."xsd:string |
| http://purl.uniprot.org/citations/16861810 | http://purl.uniprot.org/core/volume | "70"xsd:string |
| http://purl.uniprot.org/citations/16861810 | http://purl.uniprot.org/core/volume | "70"xsd:string |
| http://purl.uniprot.org/citations/16861810 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16861810 |
| http://purl.uniprot.org/citations/16861810 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16861810 |
| http://purl.uniprot.org/citations/16861810 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16861810 |
| http://purl.uniprot.org/citations/16861810 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16861810 |