Results

RDF/XMLNTriplesTurtleShow queryShare
SubjectPredicateObject
http://purl.uniprot.org/citations/16868985http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/16868985http://www.w3.org/2000/01/rdf-schema#comment"

Objective

Allograft inflammatory factor 1 (AIF-1), a protein initially identified in chronically rejected rat cardiac allografts, is involved in the immune response and proliferative vasculopathy that occurs during allograft rejection. Three well-characterized isoforms of AIF-1 result from alternative messenger RNA (mRNA) splicing. We previously identified a strong association of systemic sclerosis (SSc) with a polymorphism in AIF-1 isoform 2. The purpose of this study was to investigate AIF-1 expression in affected tissues from patients with SSc and to examine the regulation of its isoforms by transforming growth factor beta (TGFbeta).

Methods

AIF-1 in the skin and lung tissues of patients with SSc was analyzed by immunochemistry. AIF-1 isoform expression in response to TGFbeta and interferon-gamma stimulation was examined by quantitative polymerase chain reaction (PCR).

Results

AIF-1 protein was present in affected vessels of the lung and skin lesions of patients with SSc. Quantitative PCR showed an average of 14-fold higher mRNA levels in affected SSc skin than in normal skin. Double-label immunofluorescence staining demonstrated that T cells, macrophages, and endothelial cells in affected tissues expressed AIF-1. Stimulation of peripheral blood mononuclear cells with TGFbeta caused a specific and significant increase in the expression of AIF-1 isoform 2 transcripts (P < 0.005), which was due to stabilization of AIF-1 isoform 2 mRNA.

Conclusion

These data suggest that AIF-1 plays an important role in the pathogenesis of SSc owing to its increased expression in affected tissues and to the specific stimulation of AIF-1 isoform 2 by TGFbeta."xsd:string
http://purl.uniprot.org/citations/16868985http://purl.org/dc/terms/identifier"doi:10.1002/art.22010"xsd:string
http://purl.uniprot.org/citations/16868985http://purl.uniprot.org/core/author"Del Galdo F."xsd:string
http://purl.uniprot.org/citations/16868985http://purl.uniprot.org/core/author"Jimenez S.A."xsd:string
http://purl.uniprot.org/citations/16868985http://purl.uniprot.org/core/author"Maul G.G."xsd:string
http://purl.uniprot.org/citations/16868985http://purl.uniprot.org/core/author"Artlett C.M."xsd:string
http://purl.uniprot.org/citations/16868985http://purl.uniprot.org/core/date"2006"xsd:gYear
http://purl.uniprot.org/citations/16868985http://purl.uniprot.org/core/name"Arthritis Rheum"xsd:string
http://purl.uniprot.org/citations/16868985http://purl.uniprot.org/core/pages"2616-2625"xsd:string
http://purl.uniprot.org/citations/16868985http://purl.uniprot.org/core/title"Expression of allograft inflammatory factor 1 in tissues from patients with systemic sclerosis and in vitro differential expression of its isoforms in response to transforming growth factor beta."xsd:string
http://purl.uniprot.org/citations/16868985http://purl.uniprot.org/core/volume"54"xsd:string
http://purl.uniprot.org/citations/16868985http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/16868985
http://purl.uniprot.org/citations/16868985http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/16868985
http://purl.uniprot.org/uniprot/#_A1E2H8-mappedCitation-16868985http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/16868985
http://purl.uniprot.org/uniprot/#_O70200-mappedCitation-16868985http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/16868985
http://purl.uniprot.org/uniprot/#_Q4FJL9-mappedCitation-16868985http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/16868985
http://purl.uniprot.org/uniprot/#_Q9EQW9-mappedCitation-16868985http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/16868985
http://purl.uniprot.org/uniprot/#_Q7TSW1-mappedCitation-16868985http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/16868985
http://purl.uniprot.org/uniprot/Q7TSW1http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/16868985
http://purl.uniprot.org/uniprot/A1E2H8http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/16868985
http://purl.uniprot.org/uniprot/Q9EQW9http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/16868985
http://purl.uniprot.org/uniprot/O70200http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/16868985
http://purl.uniprot.org/uniprot/Q4FJL9http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/16868985