| http://purl.uniprot.org/citations/16890222 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16890222 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16890222 | http://www.w3.org/2000/01/rdf-schema#comment | "Human EML4 (EMAP-like protein 4) is a novel microtubule-associated WD-repeat protein of 120 kDa molecular weight, which is classified as belonging to the conserved family of EMAP-like proteins. Cosedimentation assays demonstrated that EML4 associates with in vitro polymerized microtubules. Correspondingly, immunofluorescence stainings and transient expression of EGFP-labeled EML4 revealed a complete colocalization of EML4 with the interphase microtubule array of HeLa cells. We present evidence that the amino-terminal portion of EML4 (amino acids 1-249) is essential for the association with microtubules. Immunoprecipitation experiments revealed that EML4 is hyperphosphorylated on serine/threonine residues during mitosis. In addition, immunofluorescence stainings demonstrated that hyperphosphorylated EML4 is associated with the mitotic spindle, suggesting that the function of EML4 is regulated by phosphorylation. siRNA-mediated knockdown of EML4 in HeLa cells led to a significant decrease in the number of cells. In no case mitotic figures could be observed in EML4 negative HeLa cells. Additionally, we observed a significant reduction of the proliferation rate and the uptake of radioactive [3H]-thymidine as a result of EML4 silencing. Most importantly, EML4 negative cells showed a completely modified microtubule network, indicating that EML4 is necessary for correct microtubule formation."xsd:string |
| http://purl.uniprot.org/citations/16890222 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.yexcr.2006.06.035"xsd:string |
| http://purl.uniprot.org/citations/16890222 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.yexcr.2006.06.035"xsd:string |
| http://purl.uniprot.org/citations/16890222 | http://purl.uniprot.org/core/author | "Buck F."xsd:string |
| http://purl.uniprot.org/citations/16890222 | http://purl.uniprot.org/core/author | "Buck F."xsd:string |
| http://purl.uniprot.org/citations/16890222 | http://purl.uniprot.org/core/author | "Kruse M.L."xsd:string |
| http://purl.uniprot.org/citations/16890222 | http://purl.uniprot.org/core/author | "Kruse M.L."xsd:string |
| http://purl.uniprot.org/citations/16890222 | http://purl.uniprot.org/core/author | "Adam-Klages S."xsd:string |
| http://purl.uniprot.org/citations/16890222 | http://purl.uniprot.org/core/author | "Adam-Klages S."xsd:string |
| http://purl.uniprot.org/citations/16890222 | http://purl.uniprot.org/core/author | "Heidebrecht H.J."xsd:string |
| http://purl.uniprot.org/citations/16890222 | http://purl.uniprot.org/core/author | "Heidebrecht H.J."xsd:string |
| http://purl.uniprot.org/citations/16890222 | http://purl.uniprot.org/core/author | "Parwaresch R."xsd:string |
| http://purl.uniprot.org/citations/16890222 | http://purl.uniprot.org/core/author | "Parwaresch R."xsd:string |
| http://purl.uniprot.org/citations/16890222 | http://purl.uniprot.org/core/author | "Pollmann M."xsd:string |
| http://purl.uniprot.org/citations/16890222 | http://purl.uniprot.org/core/author | "Pollmann M."xsd:string |
| http://purl.uniprot.org/citations/16890222 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16890222 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16890222 | http://purl.uniprot.org/core/name | "Exp. Cell Res."xsd:string |
| http://purl.uniprot.org/citations/16890222 | http://purl.uniprot.org/core/name | "Exp. Cell Res."xsd:string |
| http://purl.uniprot.org/citations/16890222 | http://purl.uniprot.org/core/pages | "3241-3251"xsd:string |
| http://purl.uniprot.org/citations/16890222 | http://purl.uniprot.org/core/pages | "3241-3251"xsd:string |
| http://purl.uniprot.org/citations/16890222 | http://purl.uniprot.org/core/title | "Human EML4, a novel member of the EMAP family, is essential for microtubule formation."xsd:string |
| http://purl.uniprot.org/citations/16890222 | http://purl.uniprot.org/core/title | "Human EML4, a novel member of the EMAP family, is essential for microtubule formation."xsd:string |