| http://purl.uniprot.org/citations/16901605 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16901605 | http://www.w3.org/2000/01/rdf-schema#comment | "In this study, we measured and characterized the bifunctional effects of a newly identified natural compound-bisindigotin (SLY-1), isolated from leaf extracts of Isatis indigotica, to CYP1A1/EROD activities in H4IIE cells. The compound, SLY-1 (1muM) elicited a transitory and significant induction of CYP1A1 RNA/protein levels and EROD activities in the cells. Maximum levels of CYP1A1 expression and EROD induction were attained at 8 and 12h of post-treatment, respectively. Thereafter the induction decreased significantly. Similar profile of CYP1A2 and CYP1B1 mRNA induction was observed. In contrast TCDD elicited CYP1A1/EROD induction was persistent. The transitory effect by SLY-1 is most likely due to the clearance of SLY-1 by cellular metabolism. Taken together the observation indicated that SLY-1 is an Ah receptor agonist for CYP1A1/CYP1A2/CYP1B1/EROD induction. Interestingly in the TCDD/SLY-1 cotreatment study, although synergistic effects on CYP1A1 expression and EROD induction were observed at 4-8h, significant inhibitory effects to TCDD induced CYP1A1 protein and EROD activity were detected at 12-24h of post-treatment. Because there was no significant reduction of CYP1A1, CYP1A2 or CYP1B1 transcript levels between TCDD- and TCDD/SLY-1 treated cells, the data pointed to the translational and/or post-translational inhibitory effect. The cellular signal transduction system may be modulated following exposure to SLY-1. To investigate the possible mechanisms involved, various specific kinase inhibitors or activators (chelerythrin, PD98059, U0126, ZM336372, SB202190, PKA inhibitor PKI (6-22) amide, and dbcAMP) were used for the assessment. Chelerythrine, PD98059 or dbcAMP treatment in TCDD induced cells showed significant inhibitory effects on CYP1A1 mRNA/protein expressions and EROD activities. U0126 had no observable EROD inhibitory effect. ZM336372 or SB202190 showed inhibition only at EROD activities. The results indicated that the SLY-1 inhibitory effect was possibly not mediated by the cAMP/PKA, PKC or MEK pathways. Nevertheless our results indicate that SLY-1 is not only an inducer of the CYP1A1 system, but also a potent inhibitor of CYP1A1 enzyme."xsd:string |
| http://purl.uniprot.org/citations/16901605 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.tox.2006.06.016"xsd:string |
| http://purl.uniprot.org/citations/16901605 | http://purl.uniprot.org/core/author | "Wei X."xsd:string |
| http://purl.uniprot.org/citations/16901605 | http://purl.uniprot.org/core/author | "Wong C.K."xsd:string |
| http://purl.uniprot.org/citations/16901605 | http://purl.uniprot.org/core/author | "Wong M.H."xsd:string |
| http://purl.uniprot.org/citations/16901605 | http://purl.uniprot.org/core/author | "Lai K.P."xsd:string |
| http://purl.uniprot.org/citations/16901605 | http://purl.uniprot.org/core/author | "Wong R.N."xsd:string |
| http://purl.uniprot.org/citations/16901605 | http://purl.uniprot.org/core/author | "Mak N.K."xsd:string |
| http://purl.uniprot.org/citations/16901605 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16901605 | http://purl.uniprot.org/core/name | "Toxicology"xsd:string |
| http://purl.uniprot.org/citations/16901605 | http://purl.uniprot.org/core/pages | "188-196"xsd:string |
| http://purl.uniprot.org/citations/16901605 | http://purl.uniprot.org/core/title | "Bifunctional modulating effects of an indigo dimer (bisindigotin) to CYP1A1 induction in H4IIE cells."xsd:string |
| http://purl.uniprot.org/citations/16901605 | http://purl.uniprot.org/core/volume | "226"xsd:string |
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