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http://purl.uniprot.org/citations/16912123http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/16912123http://www.w3.org/2000/01/rdf-schema#comment"

Context

The alteration of protein expression in white adipose tissue (WAT) may contribute to the pathogenesis of obesity.

Objective

The aim of the present study was to uncover proteins differentially expressed in the WAT of overweight/obese subjects and study the role of the identified proteins in adipocyte differentiation.

Design and setting

Two-dimensional electrophoresis and matrix-assisted laser desorption ionization-time of flight-mass spectrometry were used to identify proteins differentially expressed in WAT between obese/overweight and control groups. Cathepsin K (CTSK), one of the proteins identified by the above methods, was highlighted to assess its effects on adipocyte differentiation through 3T3-L1 cell line.

Results

Human visceral adipose tissue of overweight/obese subjects displayed a differential protein expression profile, compared with that of normal-weight controls. CTSK was up-regulated in the WAT of overweight/obese subjects, and it had a significant positive correlation with body mass index. In vitro study showed that CTSK expression and its enzyme activity gradually increased in the process of adipocyte differentiation. Moreover, E-64, an inhibitor of CTSK, could prevent adipocyte differentiation in a dose-dependent manner, which was characterized by the absence of triglyceride accumulation and glycerol contents.

Conclusions

CTSK, a cysteine protease involved in extracellular matrix remodeling, could be one of the determinants of adipocyte differentiation. CTSK may be involved in the pathogenesis of obesity by promoting adipocyte differentiation."xsd:string
http://purl.uniprot.org/citations/16912123http://purl.org/dc/terms/identifier"doi:10.1210/jc.2005-2486"xsd:string
http://purl.uniprot.org/citations/16912123http://purl.uniprot.org/core/author"Guo L."xsd:string
http://purl.uniprot.org/citations/16912123http://purl.uniprot.org/core/author"Lu W."xsd:string
http://purl.uniprot.org/citations/16912123http://purl.uniprot.org/core/author"Xiao Y."xsd:string
http://purl.uniprot.org/citations/16912123http://purl.uniprot.org/core/author"Yu Z."xsd:string
http://purl.uniprot.org/citations/16912123http://purl.uniprot.org/core/author"Min L."xsd:string
http://purl.uniprot.org/citations/16912123http://purl.uniprot.org/core/author"Sheng Z."xsd:string
http://purl.uniprot.org/citations/16912123http://purl.uniprot.org/core/author"Junfeng H."xsd:string
http://purl.uniprot.org/citations/16912123http://purl.uniprot.org/core/author"Shulin C."xsd:string
http://purl.uniprot.org/citations/16912123http://purl.uniprot.org/core/author"Tianhong L."xsd:string
http://purl.uniprot.org/citations/16912123http://purl.uniprot.org/core/author"Weixia J."xsd:string
http://purl.uniprot.org/citations/16912123http://purl.uniprot.org/core/author"Xiaohua L."xsd:string
http://purl.uniprot.org/citations/16912123http://purl.uniprot.org/core/author"Yanyun G."xsd:string
http://purl.uniprot.org/citations/16912123http://purl.uniprot.org/core/date"2006"xsd:gYear
http://purl.uniprot.org/citations/16912123http://purl.uniprot.org/core/name"J Clin Endocrinol Metab"xsd:string
http://purl.uniprot.org/citations/16912123http://purl.uniprot.org/core/pages"4520-4527"xsd:string
http://purl.uniprot.org/citations/16912123http://purl.uniprot.org/core/title"Cathepsin K in adipocyte differentiation and its potential role in the pathogenesis of obesity."xsd:string
http://purl.uniprot.org/citations/16912123http://purl.uniprot.org/core/volume"91"xsd:string
http://purl.uniprot.org/citations/16912123http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/16912123
http://purl.uniprot.org/citations/16912123http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/16912123
http://purl.uniprot.org/uniprot/#_A0A7I2YQX0-mappedCitation-16912123http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/16912123
http://purl.uniprot.org/uniprot/#_P43235-mappedCitation-16912123http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/16912123
http://purl.uniprot.org/uniprot/#_H7CEH5-mappedCitation-16912123http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/16912123
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