| http://purl.uniprot.org/citations/16916925 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16916925 | http://www.w3.org/2000/01/rdf-schema#comment | "The contribution of the POU domain, class 5, transcription factor-1 (POU5F1) in maintaining totipotency in human embryonic stem cells (hESCs) has been repeatedly proven. In humans, two isoforms are encoded: POU5F1_iA and POU5F1_iB. So far, no discrimination has been made between the isoforms in POU5F1 studies, and it is unknown which isoform contributes to the undifferentiated phenotype. Using immunocytochemistry, expression of POU5F1_iA and POU5F1_iB was examined in hESCs and all stages of human preimplantation development to look for differences in expression, biological activity, and relation to totipotency. POU5F1_iA and POU5F1_iB displayed different temporal and spatial expression patterns. During human preimplantation development, a significant POU5F1_iA expression was seen in all nuclei of compacted embryos and blastocysts and a clear POU5F1_iB expression was detected from the four-cell stage onwards in the cytoplasm of all cells. The cytoplasmic localization might imply no or other biological functions beyond transcription activation for POU5F1_iB. The stemness properties of POU5F1 can be assigned to POU5F1_iA because hESCs expressed POU5F1_iA but not POU5F1_iB. However, POU5F1_iA is not the appropriate marker to identify totipotent cells, because POU5F1_iA was also expressed in the nontotipotent trophectoderm and was not expressed in zygotes and early cleavage stage embryos, which are assumed to be totipotent. The expression pattern of POU5F1_iA may suggest that POU5F1_iA alone cannot sustain totipotency and that coexpression with other stemness factors might be the key to totipotency."xsd:string |
| http://purl.uniprot.org/citations/16916925 | http://purl.org/dc/terms/identifier | "doi:10.1634/stemcells.2005-0611"xsd:string |
| http://purl.uniprot.org/citations/16916925 | http://purl.uniprot.org/core/author | "Liebaers I."xsd:string |
| http://purl.uniprot.org/citations/16916925 | http://purl.uniprot.org/core/author | "Van Steirteghem A."xsd:string |
| http://purl.uniprot.org/citations/16916925 | http://purl.uniprot.org/core/author | "Cauffman G."xsd:string |
| http://purl.uniprot.org/citations/16916925 | http://purl.uniprot.org/core/author | "Van de Velde H."xsd:string |
| http://purl.uniprot.org/citations/16916925 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16916925 | http://purl.uniprot.org/core/name | "Stem Cells"xsd:string |
| http://purl.uniprot.org/citations/16916925 | http://purl.uniprot.org/core/pages | "2685-2691"xsd:string |
| http://purl.uniprot.org/citations/16916925 | http://purl.uniprot.org/core/title | "POU5F1 isoforms show different expression patterns in human embryonic stem cells and preimplantation embryos."xsd:string |
| http://purl.uniprot.org/citations/16916925 | http://purl.uniprot.org/core/volume | "24"xsd:string |
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