| http://purl.uniprot.org/citations/16920955 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16920955 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16920955 | http://www.w3.org/2000/01/rdf-schema#comment | "Engagement of NTB-A on human NK cells by homophilic interaction with NTB-A-expressing target cells can trigger NK cell cytotoxicity, cytokine production, and proliferation. To better understand how NTB-A can activate NK cells, we analyzed the molecular mechanisms of NTB-A signaling. We show that NTB-A is tyrosine phosphorylated in unstimulated human NK cells and associates with SLAM-associated protein (SAP) and EAT-2. This phosphorylation of NTB-A is mediated by Src family kinases and is most likely a result of the homophilic interaction of NTB-A among neighboring NK cells. Stimulation of NK cells by NTB-A-positive targets results in increased NTB-A phosphorylation. The cytoplasmic tail of NTB-A contains three tyrosines, two of which are embedded within an immunoreceptor tyrosine-based switch motif. We generated a NTB-A-negative NK cell line, in which we expressed different mutants of NTB-A. Functional studies showed that the second tyrosine is sufficient and essential for NTB-A-mediated cytotoxicity. EAT-2, but not SAP, is recruited to this second tyrosine, indicating that SAP may be dispensable for this NTB-A function. To further investigate this, we silenced SAP expression in NK cell lines. Functional analysis of these cells showed that NTB-A can mediate NK cell cytotoxicity in the absence of SAP, probably via EAT-2. In contrast, NTB-A-mediated IFN-gamma production was greatly reduced in the absence of SAP, demonstrating that cytokine production and cytotoxicity are differentially dependent on SAP and possibly EAT-2."xsd:string |
| http://purl.uniprot.org/citations/16920955 | http://purl.org/dc/terms/identifier | "doi:10.4049/jimmunol.177.5.3170"xsd:string |
| http://purl.uniprot.org/citations/16920955 | http://purl.org/dc/terms/identifier | "doi:10.4049/jimmunol.177.5.3170"xsd:string |
| http://purl.uniprot.org/citations/16920955 | http://purl.uniprot.org/core/author | "Watzl C."xsd:string |
| http://purl.uniprot.org/citations/16920955 | http://purl.uniprot.org/core/author | "Watzl C."xsd:string |
| http://purl.uniprot.org/citations/16920955 | http://purl.uniprot.org/core/author | "Eissmann P."xsd:string |
| http://purl.uniprot.org/citations/16920955 | http://purl.uniprot.org/core/author | "Eissmann P."xsd:string |
| http://purl.uniprot.org/citations/16920955 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16920955 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16920955 | http://purl.uniprot.org/core/name | "J. Immunol."xsd:string |
| http://purl.uniprot.org/citations/16920955 | http://purl.uniprot.org/core/name | "J. Immunol."xsd:string |
| http://purl.uniprot.org/citations/16920955 | http://purl.uniprot.org/core/pages | "3170-3177"xsd:string |
| http://purl.uniprot.org/citations/16920955 | http://purl.uniprot.org/core/pages | "3170-3177"xsd:string |
| http://purl.uniprot.org/citations/16920955 | http://purl.uniprot.org/core/title | "Molecular analysis of NTB-A signaling: a role for EAT-2 in NTB-A-mediated activation of human NK cells."xsd:string |
| http://purl.uniprot.org/citations/16920955 | http://purl.uniprot.org/core/title | "Molecular analysis of NTB-A signaling: a role for EAT-2 in NTB-A-mediated activation of human NK cells."xsd:string |
| http://purl.uniprot.org/citations/16920955 | http://purl.uniprot.org/core/volume | "177"xsd:string |
| http://purl.uniprot.org/citations/16920955 | http://purl.uniprot.org/core/volume | "177"xsd:string |
| http://purl.uniprot.org/citations/16920955 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16920955 |
| http://purl.uniprot.org/citations/16920955 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16920955 |
| http://purl.uniprot.org/citations/16920955 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16920955 |
| http://purl.uniprot.org/citations/16920955 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16920955 |
| http://purl.uniprot.org/uniprot/Q96DU3 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/16920955 |
| http://purl.uniprot.org/uniprot/Q96DU3#attribution-1D5652DC53099B7A14C7541DF2C66AA4 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/16920955 |