| http://purl.uniprot.org/citations/16927309 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16927309 | http://www.w3.org/2000/01/rdf-schema#comment | "The Runx2/Cbfa1 transcription factor is a scaffolding protein that promotes osteoblast differentiation; however, the specific Runx2-functional domains required for induction of the osteogenic lineage remain to be identified. We approached this question using a TERT-immortalized cell line derived from calvaria of Runx2-null mice by reconstituting the osteogenic activity with wild-type and deletion mutants of Runx2. The presence or absence of osteogenic media (beta-glycerol phosphate and ascorbic acid) and/or with BMP2 did not stimulate osteoblastic gene expression in the Runx2-null cells. However, cells infected with wild-type Runx2 adenovirus showed a robust temporal increase in the expression of osteoblast marker genes and were competent to respond to BMP2. Early markers (i.e., collagen type-1, alkaline phosphatase) were induced (four-to eightfold) at Days 4 and 8 of culture. Genes representing mature osteoblasts (e.g., Runx2, osteopontin, bone sialoprotein, osteocalcin) were temporally expressed and induced from 18-to 36-fold at Days 8 and 12. Interestingly, TGFbeta and Vitamin D-mediated transcription of osteoblast genes (except for osteopontin) required the presence of Runx2. Runx2 lacking the C-terminal 96 amino acids (Runx2 Delta432) showed a pattern of gene expression similar to wild-type protein, demonstrating the Groucho interaction and part of the activation domain are dispensable for Runx2 osteogenic activity. Upon further deletion of the Runx2 C-terminus containing the nuclear matrix targeting signal and Smad-interacting domain (Delta391), we find none of the osteoblast markers are expressed. Therefore, the Runx2 391-432 domain is essential for execution of the BMP2 osteogenic signal."xsd:string |
| http://purl.uniprot.org/citations/16927309 | http://purl.org/dc/terms/identifier | "doi:10.1002/jcb.21039"xsd:string |
| http://purl.uniprot.org/citations/16927309 | http://purl.uniprot.org/core/author | "Stein G.S."xsd:string |
| http://purl.uniprot.org/citations/16927309 | http://purl.uniprot.org/core/author | "Stein J.L."xsd:string |
| http://purl.uniprot.org/citations/16927309 | http://purl.uniprot.org/core/author | "van Wijnen A.J."xsd:string |
| http://purl.uniprot.org/citations/16927309 | http://purl.uniprot.org/core/author | "Gutierrez S."xsd:string |
| http://purl.uniprot.org/citations/16927309 | http://purl.uniprot.org/core/author | "Javed A."xsd:string |
| http://purl.uniprot.org/citations/16927309 | http://purl.uniprot.org/core/author | "Bae J.S."xsd:string |
| http://purl.uniprot.org/citations/16927309 | http://purl.uniprot.org/core/author | "Lian J.B."xsd:string |
| http://purl.uniprot.org/citations/16927309 | http://purl.uniprot.org/core/author | "Narla R."xsd:string |
| http://purl.uniprot.org/citations/16927309 | http://purl.uniprot.org/core/author | "Pratap J."xsd:string |
| http://purl.uniprot.org/citations/16927309 | http://purl.uniprot.org/core/author | "Devados R."xsd:string |
| http://purl.uniprot.org/citations/16927309 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/16927309 | http://purl.uniprot.org/core/name | "J Cell Biochem"xsd:string |
| http://purl.uniprot.org/citations/16927309 | http://purl.uniprot.org/core/pages | "434-449"xsd:string |
| http://purl.uniprot.org/citations/16927309 | http://purl.uniprot.org/core/title | "Reconstitution of Runx2/Cbfa1-null cells identifies a requirement for BMP2 signaling through a Runx2 functional domain during osteoblast differentiation."xsd:string |
| http://purl.uniprot.org/citations/16927309 | http://purl.uniprot.org/core/volume | "100"xsd:string |
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