Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/16934145http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/16934145http://www.w3.org/2000/01/rdf-schema#comment"

Background

Many genes produce multiple transcripts due to alternative splicing or utilization of alternative transcription initiation/termination sites. This 'transcriptome expansion' is thought to increase phenotypic complexity by allowing a single locus to produce several functionally distinct proteins. However, sex, genetic and developmental variation in the representation of alternative transcripts has never been examined systematically. Here, we describe a genome-wide analysis of sex-specific expression of alternative transcripts in Drosophila melanogaster.

Results

We compared transcript profiles in males and females from eight Drosophila lines (OregonR and 2b, and 6 RIL) using a newly designed 60-mer oligonucleotide microarray that allows us to distinguish a large proportion of alternative transcripts. The new microarray incorporates 7,207 oligonucleotides, satisfying stringent binding and specificity criteria that target both the common and the unique regions of 2,768 multi-transcript genes, as well as 12,912 oligonucleotides that target genes with a single known transcript. We estimate that up to 22% of genes that produce multiple transcripts show a sex-specific bias in the representation of alternative transcripts. Sexual dimorphism in overall transcript abundance was evident for 53% of genes. The X chromosome contains a significantly higher proportion of genes with female-biased transcription than the autosomes. However, genes on the X chromosome are no more likely to have a sexual bias in alternative transcript representation than autosomal genes.

Conclusion

Widespread sex-specific expression of alternative transcripts in Drosophila suggests that a new level of sexual dimorphism at the molecular level exists."xsd:string
http://purl.uniprot.org/citations/16934145http://purl.org/dc/terms/identifier"doi:10.1186/gb-2006-7-8-r79"xsd:string
http://purl.uniprot.org/citations/16934145http://purl.uniprot.org/core/author"Westerman R."xsd:string
http://purl.uniprot.org/citations/16934145http://purl.uniprot.org/core/author"Kopp A."xsd:string
http://purl.uniprot.org/citations/16934145http://purl.uniprot.org/core/author"McIntyre L.M."xsd:string
http://purl.uniprot.org/citations/16934145http://purl.uniprot.org/core/author"Genissel A."xsd:string
http://purl.uniprot.org/citations/16934145http://purl.uniprot.org/core/author"Wayne M.L."xsd:string
http://purl.uniprot.org/citations/16934145http://purl.uniprot.org/core/author"Harshman L."xsd:string
http://purl.uniprot.org/citations/16934145http://purl.uniprot.org/core/author"Nuzhdin S.V."xsd:string
http://purl.uniprot.org/citations/16934145http://purl.uniprot.org/core/author"Telonis-Scott M."xsd:string
http://purl.uniprot.org/citations/16934145http://purl.uniprot.org/core/author"Bono L.M."xsd:string
http://purl.uniprot.org/citations/16934145http://purl.uniprot.org/core/author"Junk D."xsd:string
http://purl.uniprot.org/citations/16934145http://purl.uniprot.org/core/date"2006"xsd:gYear
http://purl.uniprot.org/citations/16934145http://purl.uniprot.org/core/name"Genome Biol"xsd:string
http://purl.uniprot.org/citations/16934145http://purl.uniprot.org/core/pages"R79"xsd:string
http://purl.uniprot.org/citations/16934145http://purl.uniprot.org/core/title"Sex-specific expression of alternative transcripts in Drosophila."xsd:string
http://purl.uniprot.org/citations/16934145http://purl.uniprot.org/core/volume"7"xsd:string
http://purl.uniprot.org/citations/16934145http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/16934145
http://purl.uniprot.org/citations/16934145http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/16934145
http://purl.uniprot.org/uniprot/#_A0A0B4KFS4-mappedCitation-16934145http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/16934145
http://purl.uniprot.org/uniprot/#_A0A0B4KF97-mappedCitation-16934145http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/16934145
http://purl.uniprot.org/uniprot/#_A0A0C4DHG5-mappedCitation-16934145http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/16934145
http://purl.uniprot.org/uniprot/#_A0A0C4DHG9-mappedCitation-16934145http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/16934145
http://purl.uniprot.org/uniprot/#_A0A0B4K6R9-mappedCitation-16934145http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/16934145
▶