| http://purl.uniprot.org/citations/16938851 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16938851 | http://www.w3.org/2000/01/rdf-schema#comment | "Matrix assembly and homeostasis in collagen-rich tissues are mediated by interactions with proteoglycans (PGs) substituted with sulfated glycosaminoglycans (GAGs). The major GAG in cornea is keratan sulfate (KS), which is N-linked to one of three PG core proteins. To ascertain the importance of the carbohydrate chain sulfation step in KS functionality, we generated a strain of mice with a targeted gene deletion in Chst5, which encodes an N-acetylglucosamine-6-O-sulfotransferase that is integral to the sulfation of KS chains. Corneas of homozygous mutants were significantly thinner than those of WT or heterozygous mice. They lacked high-sulfated KS, but contained the core protein of the major corneal KSPG, lumican. Histochemically stained KSPGs coassociated with fibrillar collagen in WT corneas, but were not identified in the Chst5-null tissue. Conversely, abnormally large chondroitin sulfate/dermatan sulfate PG complexes were abundant throughout the Chst5-deficient cornea, indicating an alteration of controlled PG production in the mutant cornea. The corneal stroma of the Chst5-null mouse exhibited widespread structural alterations in collagen fibrillar architecture, including decreased interfibrillar spacing and a more spatially disorganized collagen array. The enzymatic sulfation of KS GAG chains is thus identified as a key requirement for PG biosynthesis and collagen matrix organization."xsd:string |
| http://purl.uniprot.org/citations/16938851 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.0605441103"xsd:string |
| http://purl.uniprot.org/citations/16938851 | http://purl.uniprot.org/core/author | "Nishida K."xsd:string |
| http://purl.uniprot.org/citations/16938851 | http://purl.uniprot.org/core/author | "Nakayama J."xsd:string |
| http://purl.uniprot.org/citations/16938851 | http://purl.uniprot.org/core/author | "Tanigami A."xsd:string |
| http://purl.uniprot.org/citations/16938851 | http://purl.uniprot.org/core/author | "Kerr B."xsd:string |
| http://purl.uniprot.org/citations/16938851 | http://purl.uniprot.org/core/author | "Caterson B."xsd:string |
| http://purl.uniprot.org/citations/16938851 | http://purl.uniprot.org/core/author | "Hughes C.E."xsd:string |
| http://purl.uniprot.org/citations/16938851 | http://purl.uniprot.org/core/author | "Lewis P."xsd:string |
| http://purl.uniprot.org/citations/16938851 | http://purl.uniprot.org/core/author | "Tano Y."xsd:string |
| http://purl.uniprot.org/citations/16938851 | http://purl.uniprot.org/core/author | "Akama T.O."xsd:string |
| http://purl.uniprot.org/citations/16938851 | http://purl.uniprot.org/core/author | "Hayashida Y."xsd:string |
| http://purl.uniprot.org/citations/16938851 | http://purl.uniprot.org/core/author | "Quantock A.J."xsd:string |
| http://purl.uniprot.org/citations/16938851 | http://purl.uniprot.org/core/author | "Young R.D."xsd:string |
| http://purl.uniprot.org/citations/16938851 | http://purl.uniprot.org/core/author | "Meek K.M."xsd:string |
| http://purl.uniprot.org/citations/16938851 | http://purl.uniprot.org/core/author | "Beecher N."xsd:string |
| http://purl.uniprot.org/citations/16938851 | http://purl.uniprot.org/core/author | "Fukada M.N."xsd:string |
| http://purl.uniprot.org/citations/16938851 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16938851 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/16938851 | http://purl.uniprot.org/core/pages | "13333-13338"xsd:string |
| http://purl.uniprot.org/citations/16938851 | http://purl.uniprot.org/core/title | "Matrix morphogenesis in cornea is mediated by the modification of keratan sulfate by GlcNAc 6-O-sulfotransferase."xsd:string |
| http://purl.uniprot.org/citations/16938851 | http://purl.uniprot.org/core/volume | "103"xsd:string |
| http://purl.uniprot.org/citations/16938851 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16938851 |
| http://purl.uniprot.org/citations/16938851 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16938851 |