| http://purl.uniprot.org/citations/16940963 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16940963 | http://www.w3.org/2000/01/rdf-schema#comment | "Members of the Rho family of GTPases are key regulators of the actin cytoskeleton. In particular, activated Rac1 stimulates membrane dorsal ruffle formation in response to platelet-derived growth factor (PDGF). Abl-interactor (Abi)-1 and betaPIX, a guanine nucleotide exchange factor for Rac1, localise at these Rac1-induced actin structures and play important roles in the induction of membrane dorsal ruffling in response to PDGF in fibroblasts. Here, we demonstrate a novel interaction between Abi-1 and betaPIX using the yeast two-hybrid system, in vitro pull-down assays, and in vivo co-immunoprecipitation experiments. In vitro, the C-terminal fragment of betaPIX interacted with Abi-1, while in vivo the N-terminal fragment of betaPIX interacted with Abi-1. The biological function of this interaction was investigated in mouse fibroblasts in response to PDGF stimulation. Abi-1 and betaPIX co-localised in the cytoplasm and to membrane dorsal ruffles after PDGF treatment. We show that the co-expression of Abi-1 and truncated forms of betaPIX in mouse fibroblasts blocked PDGF-induced membrane dorsal ruffles. Together, these results show that the interaction between Abi-1 and betaPIX is involved in the formation of growth factor-induced membrane dorsal ruffles."xsd:string |
| http://purl.uniprot.org/citations/16940963 | http://purl.org/dc/terms/identifier | "doi:10.1038/sj.cr.7310091"xsd:string |
| http://purl.uniprot.org/citations/16940963 | http://purl.uniprot.org/core/author | "Klein A."xsd:string |
| http://purl.uniprot.org/citations/16940963 | http://purl.uniprot.org/core/author | "Rudel T."xsd:string |
| http://purl.uniprot.org/citations/16940963 | http://purl.uniprot.org/core/author | "Machuy N."xsd:string |
| http://purl.uniprot.org/citations/16940963 | http://purl.uniprot.org/core/author | "Campa F."xsd:string |
| http://purl.uniprot.org/citations/16940963 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16940963 | http://purl.uniprot.org/core/name | "Cell Res"xsd:string |
| http://purl.uniprot.org/citations/16940963 | http://purl.uniprot.org/core/pages | "759-770"xsd:string |
| http://purl.uniprot.org/citations/16940963 | http://purl.uniprot.org/core/title | "A new interaction between Abi-1 and betaPIX involved in PDGF-activated actin cytoskeleton reorganisation."xsd:string |
| http://purl.uniprot.org/citations/16940963 | http://purl.uniprot.org/core/volume | "16"xsd:string |
| http://purl.uniprot.org/citations/16940963 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16940963 |
| http://purl.uniprot.org/citations/16940963 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16940963 |
| http://purl.uniprot.org/uniprot/#_A0A0R4J0X8-mappedCitation-16940963 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16940963 |
| http://purl.uniprot.org/uniprot/#_B7ZCU0-mappedCitation-16940963 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16940963 |
| http://purl.uniprot.org/uniprot/#_B7ZCU3-mappedCitation-16940963 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16940963 |
| http://purl.uniprot.org/uniprot/#_B7ZCU4-mappedCitation-16940963 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16940963 |
| http://purl.uniprot.org/uniprot/#_Q3TJR5-mappedCitation-16940963 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16940963 |
| http://purl.uniprot.org/uniprot/#_Q3TJ64-mappedCitation-16940963 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16940963 |
| http://purl.uniprot.org/uniprot/#_Q3TMI0-mappedCitation-16940963 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16940963 |
| http://purl.uniprot.org/uniprot/#_Q3TPY5-mappedCitation-16940963 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16940963 |
| http://purl.uniprot.org/uniprot/#_Q8CBW3-mappedCitation-16940963 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16940963 |
| http://purl.uniprot.org/uniprot/#_Q3UAH6-mappedCitation-16940963 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16940963 |
| http://purl.uniprot.org/uniprot/#_Q6AXD3-mappedCitation-16940963 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16940963 |