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http://purl.uniprot.org/citations/16952964http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/16952964http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/16952964http://www.w3.org/2000/01/rdf-schema#comment"Flagellar operons are divided into three classes with respect to their transcriptional hierarchy in Salmonella enterica serovar Typhimurium. The class 1 gene products FlhD and FlhC act together in an FlhD(2)C(2) heterotetramer, which binds upstream of the class 2 promoters to facilitate binding of RNA polymerase. Class 2 expression is known to be enhanced by a disruption mutation in a flagellar gene, fliT. In this study, we purified FliT protein in a His-tagged form and showed that the protein prevented binding of FlhD(2)C(2) to the class 2 promoter and inhibited FlhD(2)C(2)-dependent transcription. Pull-down and far-Western blotting analyses revealed that the FliT protein was capable of binding to FlhD(2)C(2) and FlhC and not to FlhD alone. We conclude that FliT acts as an anti-FlhD(2)C(2) factor, which binds to FlhD(2)C(2) through interaction with the FlhC subunit and inhibits its binding to the class 2 promoter."xsd:string
http://purl.uniprot.org/citations/16952964http://purl.org/dc/terms/identifier"doi:10.1128/jb.00799-06"xsd:string
http://purl.uniprot.org/citations/16952964http://purl.org/dc/terms/identifier"doi:10.1128/jb.00799-06"xsd:string
http://purl.uniprot.org/citations/16952964http://purl.uniprot.org/core/author"Yamamoto S."xsd:string
http://purl.uniprot.org/citations/16952964http://purl.uniprot.org/core/author"Yamamoto S."xsd:string
http://purl.uniprot.org/citations/16952964http://purl.uniprot.org/core/author"Kutsukake K."xsd:string
http://purl.uniprot.org/citations/16952964http://purl.uniprot.org/core/author"Kutsukake K."xsd:string
http://purl.uniprot.org/citations/16952964http://purl.uniprot.org/core/date"2006"xsd:gYear
http://purl.uniprot.org/citations/16952964http://purl.uniprot.org/core/date"2006"xsd:gYear
http://purl.uniprot.org/citations/16952964http://purl.uniprot.org/core/name"J. Bacteriol."xsd:string
http://purl.uniprot.org/citations/16952964http://purl.uniprot.org/core/name"J. Bacteriol."xsd:string
http://purl.uniprot.org/citations/16952964http://purl.uniprot.org/core/pages"6703-6708"xsd:string
http://purl.uniprot.org/citations/16952964http://purl.uniprot.org/core/pages"6703-6708"xsd:string
http://purl.uniprot.org/citations/16952964http://purl.uniprot.org/core/title"FliT acts as an anti-FlhD2C2 factor in the transcriptional control of the flagellar regulon in Salmonella enterica serovar typhimurium."xsd:string
http://purl.uniprot.org/citations/16952964http://purl.uniprot.org/core/title"FliT acts as an anti-FlhD2C2 factor in the transcriptional control of the flagellar regulon in Salmonella enterica serovar typhimurium."xsd:string
http://purl.uniprot.org/citations/16952964http://purl.uniprot.org/core/volume"188"xsd:string
http://purl.uniprot.org/citations/16952964http://purl.uniprot.org/core/volume"188"xsd:string
http://purl.uniprot.org/citations/16952964http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/16952964
http://purl.uniprot.org/citations/16952964http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/16952964
http://purl.uniprot.org/citations/16952964http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/16952964
http://purl.uniprot.org/citations/16952964http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/16952964
http://purl.uniprot.org/uniprot/P0A1N2http://purl.uniprot.org/core/citationhttp://purl.uniprot.org/citations/16952964
http://purl.uniprot.org/uniprot/P0A1N2#attribution-12B0FD105DAF8DA98D1EB720A4D35649http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/16952964