| http://purl.uniprot.org/citations/16960125 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16960125 | http://www.w3.org/2000/01/rdf-schema#comment | "Thyroid transcription factor-1 (TTF-1, product of the Nkx2.1 gene) is essential for branching morphogenesis of the lung and enhances expression of surfactant proteins by alveolar type II cells. We investigated expression of two TTF-1 mRNA transcripts, generated by alternative start sites and coding for 42- and 46-kD protein isoforms in the mouse, during hormone-induced differentiation of human fetal lung type II cells in culture. Transcript for 42-kD TTF-1 was 20-fold more abundant than TTF-1(46) mRNA by RT-PCR. Only 42-kD protein was detected in lung cells, and its content increased during in vivo development and in response to in vitro glucocorticoid plus cAMP treatment. To examine TTF-1 target proteins, recombinant, phosphorylated TTF-1(42) was expressed in nuclei of cells by adenovirus transduction. By microarray analysis, 14 genes were comparably induced by recombinant TTF-1 (rTTF-1) and hormone treatment, and 9 additional hormone-responsive genes, including surfactant proteins-A/B/C, were partially induced by rTTF-1. The most highly (approximately 10-fold) TTF-1-induced genes were DC-LAMP (LAMP3) and CEACAM6 with induction confirmed by Western analysis and immunostaining. Treatment of cells with hormones plus small inhibitory RNA directed toward TTF-1 reduced TTF-1 content by approximately 50% and inhibited hormone induction of the 23 genes induced by rTTF-1. In addition, knockdown of TTF-1 inhibited 72 of 274 other genes induced by hormones. We conclude that 42-kD TTF-1 is required for induction of a subset of regulated genes during type II cell differentiation."xsd:string |
| http://purl.uniprot.org/citations/16960125 | http://purl.org/dc/terms/identifier | "doi:10.1165/rcmb.2006-0207oc"xsd:string |
| http://purl.uniprot.org/citations/16960125 | http://purl.uniprot.org/core/author | "Wang P."xsd:string |
| http://purl.uniprot.org/citations/16960125 | http://purl.uniprot.org/core/author | "Ballard P.L."xsd:string |
| http://purl.uniprot.org/citations/16960125 | http://purl.uniprot.org/core/author | "Feinstein S.I."xsd:string |
| http://purl.uniprot.org/citations/16960125 | http://purl.uniprot.org/core/author | "Gonzales L.W."xsd:string |
| http://purl.uniprot.org/citations/16960125 | http://purl.uniprot.org/core/author | "Kolla V."xsd:string |
| http://purl.uniprot.org/citations/16960125 | http://purl.uniprot.org/core/author | "Gonzales J."xsd:string |
| http://purl.uniprot.org/citations/16960125 | http://purl.uniprot.org/core/author | "Angampalli S."xsd:string |
| http://purl.uniprot.org/citations/16960125 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/16960125 | http://purl.uniprot.org/core/name | "Am J Respir Cell Mol Biol"xsd:string |
| http://purl.uniprot.org/citations/16960125 | http://purl.uniprot.org/core/pages | "213-225"xsd:string |
| http://purl.uniprot.org/citations/16960125 | http://purl.uniprot.org/core/title | "Thyroid transcription factor in differentiating type II cells: regulation, isoforms, and target genes."xsd:string |
| http://purl.uniprot.org/citations/16960125 | http://purl.uniprot.org/core/volume | "36"xsd:string |
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