| http://purl.uniprot.org/citations/16973378 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16973378 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16973378 | http://www.w3.org/2000/01/rdf-schema#comment | "The full length human adenylosuccinate lyase gene was generated by a PCR method using a plasmid encoding a truncated human enzyme as template, and was cloned into a pET-14b vector. Human adenylosuccinate lyase was overexpressed in Escherichia coli Rosetta 2(DE3)pLysS as an N-terminal histidine-tagged protein and was purified to homogeneity by a nickel-nitriloacetic acid column at room temperature. The histidine tag was removed from the human enzyme by thrombin digestion and the adenylosuccinate lyase was purified by Sephadex G-100 gel filtration. The histidine-tagged and non-tagged adenylosuccinate lyases exhibit similar values of Vmax and Km for S-AMP. Analytical ultracentrifugation and circular dichroism revealed, respectively, that the histidine-tagged enzyme is in tetrameric form with a molecular weight of 220 kDa and contains predominantly alpha-helical structure. This is the first purification procedure to yield a stable form of human adenylosuccinate lyase. The enzyme is stable for at least 5 days at 25 degrees C, and upon rapid freezing and thawing. Temperature as well as reducing agent (DTT) play critical roles in determining the stability of the human adenylosuccinate lyase."xsd:string |
| http://purl.uniprot.org/citations/16973378 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.pep.2006.07.023"xsd:string |
| http://purl.uniprot.org/citations/16973378 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.pep.2006.07.023"xsd:string |
| http://purl.uniprot.org/citations/16973378 | http://purl.uniprot.org/core/author | "Lee P."xsd:string |
| http://purl.uniprot.org/citations/16973378 | http://purl.uniprot.org/core/author | "Lee P."xsd:string |
| http://purl.uniprot.org/citations/16973378 | http://purl.uniprot.org/core/author | "Colman R.F."xsd:string |
| http://purl.uniprot.org/citations/16973378 | http://purl.uniprot.org/core/author | "Colman R.F."xsd:string |
| http://purl.uniprot.org/citations/16973378 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/16973378 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/16973378 | http://purl.uniprot.org/core/name | "Protein Expr. Purif."xsd:string |
| http://purl.uniprot.org/citations/16973378 | http://purl.uniprot.org/core/name | "Protein Expr. Purif."xsd:string |
| http://purl.uniprot.org/citations/16973378 | http://purl.uniprot.org/core/pages | "227-234"xsd:string |
| http://purl.uniprot.org/citations/16973378 | http://purl.uniprot.org/core/pages | "227-234"xsd:string |
| http://purl.uniprot.org/citations/16973378 | http://purl.uniprot.org/core/title | "Expression, purification, and characterization of stable, recombinant human adenylosuccinate lyase."xsd:string |
| http://purl.uniprot.org/citations/16973378 | http://purl.uniprot.org/core/title | "Expression, purification, and characterization of stable, recombinant human adenylosuccinate lyase."xsd:string |
| http://purl.uniprot.org/citations/16973378 | http://purl.uniprot.org/core/volume | "51"xsd:string |
| http://purl.uniprot.org/citations/16973378 | http://purl.uniprot.org/core/volume | "51"xsd:string |
| http://purl.uniprot.org/citations/16973378 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16973378 |
| http://purl.uniprot.org/citations/16973378 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16973378 |
| http://purl.uniprot.org/citations/16973378 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16973378 |
| http://purl.uniprot.org/citations/16973378 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16973378 |
| http://purl.uniprot.org/uniprot/P30566 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/16973378 |
| http://purl.uniprot.org/uniprot/P30566#attribution-1D4635836A774215743AEB9B718B3684 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/16973378 |