| http://purl.uniprot.org/citations/16980298 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16980298 | http://www.w3.org/2000/01/rdf-schema#comment | "Immune cell function is modulated by changes in extracellular nucleotide levels. Here we used reverse transcription-PCR analyses, single cell Ca2+ imaging, and knock-out mice to define the receptors mediating nucleotide-induced Ca2+ signaling in resident peritoneal macrophages. In Ca2+-free buffer, the potent (K0.5<1 microm) stimulatory effect of UTP (or ATP) on endoplasmic reticulum (ER) Ca2+ release was abolished in cells isolated from P2Y2/P2Y4 double knock-out mice. Moreover, P2Y4(0/-), but not P2Y2-/-, macrophages responded to UTP. In P2Y2-/-macrophages, we could elicit Ca2+ responses to "pure" P2X receptor activation by applying ATP in buffer containing Ca2+. Purified UDP and ADP were ineffective agonists, although modest UDP-induced Ca2+ responses could be elicited in macrophages after "activation" with lipopolysaccharide and interferon-gamma. Notably, in Ca2+-free buffer, UTP-induced Ca2+ transients decayed within 1 min, and there was no response to repeated agonist challenge. Measurements of ER [Ca2+] with mag-fluo-4 showed that ER Ca2+ stores were depleted under these conditions. When extracellular Ca2+ was available, ER Ca2+ stores refilled, but Ca2+ increased to only approximately 40% of the initial value upon repeated UTP challenge. This apparent receptor desensitization persisted in GRK2+/- and GRK6-/-macrophages and after inhibition of candidate kinases protein kinase C and calmodulin-dependent kinase II. Initial challenge with UTP also reduced Ca2+ mobilization by complement component C5a (and vice versa). In conclusion, homologous receptor desensitization is not the major mechanism that rapidly dampens Ca2+ signaling mediated by P2Y2, the sole Gq-coupled receptor for UTP or ATP in macrophages. UDP responsiveness (P2Y6 receptor expression) increases following macrophage activation."xsd:string |
| http://purl.uniprot.org/citations/16980298 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m607713200"xsd:string |
| http://purl.uniprot.org/citations/16980298 | http://purl.uniprot.org/core/author | "Kavelaars A."xsd:string |
| http://purl.uniprot.org/citations/16980298 | http://purl.uniprot.org/core/author | "del Rey A."xsd:string |
| http://purl.uniprot.org/citations/16980298 | http://purl.uniprot.org/core/author | "Renigunta V."xsd:string |
| http://purl.uniprot.org/citations/16980298 | http://purl.uniprot.org/core/author | "Zuzarte M."xsd:string |
| http://purl.uniprot.org/citations/16980298 | http://purl.uniprot.org/core/author | "Robaye B."xsd:string |
| http://purl.uniprot.org/citations/16980298 | http://purl.uniprot.org/core/author | "Leipziger J."xsd:string |
| http://purl.uniprot.org/citations/16980298 | http://purl.uniprot.org/core/author | "Dalpke A.H."xsd:string |
| http://purl.uniprot.org/citations/16980298 | http://purl.uniprot.org/core/author | "Hanley P.J."xsd:string |
| http://purl.uniprot.org/citations/16980298 | http://purl.uniprot.org/core/author | "Matos J.E."xsd:string |
| http://purl.uniprot.org/citations/16980298 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16980298 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/16980298 | http://purl.uniprot.org/core/pages | "35147-35155"xsd:string |
| http://purl.uniprot.org/citations/16980298 | http://purl.uniprot.org/core/title | "Knock-out mice reveal the contributions of P2Y and P2X receptors to nucleotide-induced Ca2+ signaling in macrophages."xsd:string |
| http://purl.uniprot.org/citations/16980298 | http://purl.uniprot.org/core/volume | "281"xsd:string |
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