| http://purl.uniprot.org/citations/16981728 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16981728 | http://www.w3.org/2000/01/rdf-schema#comment | "The structure of the NH2-terminal region of troponin T (TnT) is hypervariable among the muscle type-specific isoforms and is also regulated by alternative RNA splicing. This region does not contain binding sites for other thin filament proteins, but alteration of its structure affects the Ca2+ regulation of muscle contraction. Here we report a truncated cardiac TnT produced during myocardial ischemia reperfusion. Amino acid sequencing and protein fragment reconstruction determined that it is generated by a posttranslational modification selectively removing the NH2-terminal variable region and preserving the conserved core structure of TnT. Triton X-100 extraction of cardiac muscle fibers promoted production of the NH2-terminal truncated cardiac TnT (cTnT-ND), indicating a myofibril-associated proteolytic activity. Mu-calpain is a myofibril-associated protease and is known to degrade TnT. Supporting a role of mu-calpain in producing cTnT-ND in myocardial ischemia reperfusion, calpain inhibitors decreased the level of cTnT-ND in Triton-extracted myofibrils. Mu-calpain treatment of the cardiac myofibril and troponin complex specifically reproduced cTnT-ND. In contrast, mu-calpain treatment of isolated cardiac TnT resulted in nonspecific degradation, suggesting that this structural modification is relevant to physiological structures of the myofilament. Triton X-100 treatment of transgenic mouse cardiac myofibrils overexpressing fast skeletal muscle TnT produced similar NH2-terminal truncations of the endogenous and exogenous TnT, despite different amino acid sequences at the cleavage site. With the functional consequences of removing the NH2-terminal variable region of TnT, the mu-calpain-mediated proteolytic modification of TnT may act as an acute mechanism to adjust muscle contractility under stress conditions."xsd:string |
| http://purl.uniprot.org/citations/16981728 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi060273s"xsd:string |
| http://purl.uniprot.org/citations/16981728 | http://purl.uniprot.org/core/author | "Zhang Z."xsd:string |
| http://purl.uniprot.org/citations/16981728 | http://purl.uniprot.org/core/author | "Jin J.P."xsd:string |
| http://purl.uniprot.org/citations/16981728 | http://purl.uniprot.org/core/author | "Biesiadecki B.J."xsd:string |
| http://purl.uniprot.org/citations/16981728 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16981728 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/16981728 | http://purl.uniprot.org/core/pages | "11681-11694"xsd:string |
| http://purl.uniprot.org/citations/16981728 | http://purl.uniprot.org/core/title | "Selective deletion of the NH2-terminal variable region of cardiac troponin T in ischemia reperfusion by myofibril-associated mu-calpain cleavage."xsd:string |
| http://purl.uniprot.org/citations/16981728 | http://purl.uniprot.org/core/volume | "45"xsd:string |
| http://purl.uniprot.org/citations/16981728 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16981728 |
| http://purl.uniprot.org/citations/16981728 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16981728 |
| http://purl.uniprot.org/uniprot/#_A0A087WQP1-mappedCitation-16981728 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16981728 |
| http://purl.uniprot.org/uniprot/#_A0A087WRX3-mappedCitation-16981728 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16981728 |
| http://purl.uniprot.org/uniprot/#_A6ICH0-mappedCitation-16981728 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16981728 |
| http://purl.uniprot.org/uniprot/#_A6ICH2-mappedCitation-16981728 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16981728 |
| http://purl.uniprot.org/uniprot/#_A0A8L2QKB8-mappedCitation-16981728 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16981728 |
| http://purl.uniprot.org/uniprot/#_A0A8L2UPK2-mappedCitation-16981728 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16981728 |
| http://purl.uniprot.org/uniprot/#_A0A494BAC4-mappedCitation-16981728 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16981728 |
| http://purl.uniprot.org/uniprot/#_A0A494BAS0-mappedCitation-16981728 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16981728 |
| http://purl.uniprot.org/uniprot/#_A0A494BB02-mappedCitation-16981728 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16981728 |
| http://purl.uniprot.org/uniprot/#_B1WBR4-mappedCitation-16981728 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16981728 |
| http://purl.uniprot.org/uniprot/#_A9YUA5-mappedCitation-16981728 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16981728 |
| http://purl.uniprot.org/uniprot/#_O35350-mappedCitation-16981728 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16981728 |