| http://purl.uniprot.org/citations/17005554 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17005554 | http://www.w3.org/2000/01/rdf-schema#comment | "We have quantified the fidelity of polymerization of DNA by human mitochondrial DNA polymerase using synthetic DNA oligonucleotides and recombinant holoenzyme and examining each of the possible 16-base pair combinations. Although the kinetics of incorporation for all correct nucleotides are similar, with an average Kd of 0.8 microM and an average k(pol) of 37 s(-1), the kinetics of misincorporation vary widely. The ground state binding Kd of incorrect bases ranges from a low of 25 microM for a dATP:A mispair to a high of 360 microM for a dCTP:T mispair. Similarly, the rates of incorporation of incorrect bases vary from 0.0031 s(-1) for a dCTP:C mispair to 1.16 s(-1) for a dGTP:T mispair. Due to the variability in the kinetic parameters for misincorporation, the estimates of fidelity range from 1 error in 3563 nucleotides for dGTP:T to 1 error in 2.3 x 10(6) nucleotides for dCTP:C. Interestingly, the discrimination against a dGTP:T mismatch is 16.5 times lower than that of a dTTP:G mismatch due to a tighter Kd for ground state binding and a faster rate of incorporation of the dGTP:T mismatch relative to the dTTP:G mismatch. We calculate an average fidelity of 1 error in 440,000 nucleotides."xsd:string |
| http://purl.uniprot.org/citations/17005554 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m607964200"xsd:string |
| http://purl.uniprot.org/citations/17005554 | http://purl.uniprot.org/core/author | "Johnson K.A."xsd:string |
| http://purl.uniprot.org/citations/17005554 | http://purl.uniprot.org/core/author | "Lee H.R."xsd:string |
| http://purl.uniprot.org/citations/17005554 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/17005554 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/17005554 | http://purl.uniprot.org/core/pages | "36236-36240"xsd:string |
| http://purl.uniprot.org/citations/17005554 | http://purl.uniprot.org/core/title | "Fidelity of the human mitochondrial DNA polymerase."xsd:string |
| http://purl.uniprot.org/citations/17005554 | http://purl.uniprot.org/core/volume | "281"xsd:string |
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