| http://purl.uniprot.org/citations/17008134 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17008134 | http://www.w3.org/2000/01/rdf-schema#comment | "JAK/STAT signaling is essential for a wide range of developmental processes in Drosophila melanogaster. The mechanism by which the JAK/STAT pathway contributes to these processes has been the subject of recent investigation. However, a reporter that reflects activity of the JAK/STAT pathway in all Drosophila tissues has not yet been developed. By placing a fragment of the Stat92E target gene Socs36E, which contains at least two putative Stat92E binding sites, upstream of GFP, we generated three constructs that can be used to monitor JAK/STAT pathway activity in vivo. These constructs differ by the number of Stat92E binding sites and the stability of GFP. The 2XSTAT92E-GFP and 10XSTAT92E-GFP constructs contain 2 and 10 Stat92E binding sites, respectively, driving expression of enhanced GFP, while 10XSTAT92E-DGFP drives expression of destabilized GFP. We show that these reporters are expressed in the embryo in an overlapping pattern with Stat92E protein and in tissues where JAK/STAT signaling is required. In addition, these reporters accurately reflect JAK/STAT pathway activity at larval stages, as their expression pattern overlaps that of the activating ligand unpaired in imaginal discs. Moreover, the STAT92E-GFP reporters are activated by ectopic JAK/STAT signaling. STAT92E-GFP fluorescence is increased in response to ectopic upd in the larval eye disc and mis-expression of the JAK kinase hopscotch in the adult fat body. Lastly, these reporters are specifically activated by Stat92E, as STAT92E-GFP reporter expression is lost cell-autonomously in stat92E homozygous mutant tissue. In sum, we have generated in vivo GFP reporters that accurately reflect JAK/STAT pathway activation in a variety of tissues. These reporters are valuable tools to further investigate and understand the role of JAK/STAT signaling in Drosophila."xsd:string |
| http://purl.uniprot.org/citations/17008134 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.modgep.2006.08.003"xsd:string |
| http://purl.uniprot.org/citations/17008134 | http://purl.uniprot.org/core/author | "Lee H."xsd:string |
| http://purl.uniprot.org/citations/17008134 | http://purl.uniprot.org/core/author | "Perrimon N."xsd:string |
| http://purl.uniprot.org/citations/17008134 | http://purl.uniprot.org/core/author | "Flaherty M.S."xsd:string |
| http://purl.uniprot.org/citations/17008134 | http://purl.uniprot.org/core/author | "Bach E.A."xsd:string |
| http://purl.uniprot.org/citations/17008134 | http://purl.uniprot.org/core/author | "Ekas L.A."xsd:string |
| http://purl.uniprot.org/citations/17008134 | http://purl.uniprot.org/core/author | "Baeg G.H."xsd:string |
| http://purl.uniprot.org/citations/17008134 | http://purl.uniprot.org/core/author | "Ayala-Camargo A."xsd:string |
| http://purl.uniprot.org/citations/17008134 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17008134 | http://purl.uniprot.org/core/name | "Gene Expr Patterns"xsd:string |
| http://purl.uniprot.org/citations/17008134 | http://purl.uniprot.org/core/pages | "323-331"xsd:string |
| http://purl.uniprot.org/citations/17008134 | http://purl.uniprot.org/core/title | "GFP reporters detect the activation of the Drosophila JAK/STAT pathway in vivo."xsd:string |
| http://purl.uniprot.org/citations/17008134 | http://purl.uniprot.org/core/volume | "7"xsd:string |
| http://purl.uniprot.org/citations/17008134 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17008134 |
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