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| http://purl.uniprot.org/citations/17008717 | http://www.w3.org/2000/01/rdf-schema#comment | "The interaction of cellular proteins with the gap junction protein Connexin43 (Cx43) is thought to form a dynamic scaffolding complex that functions as a platform for the assembly of signaling, structural, and cytoskeletal proteins. A high stringency Scansite search of rat Cx43 identified the motif containing Ser373 (S373) as a 14-3-3 binding site. The S373 motif and the second best mode-1 motif, containing Ser244 (S244), are conserved in rat, mouse, human, chicken, and bovine, but not in Xenopus or zebrafish Cx43. Docking studies of a mouse/rat 14-3-3 homology model with the modeled phosphorylated S373 or S244 peptide ligands or their serine-to-alanine mutants, S373A or S244A, revealed that the pS373 motif facilitated a greater number of intermolecular contacts than the pS244 motif, thus supporting a stronger 14-3-3 binding interaction with the pS373 motif. The alanine substitution also reduced more than half the number of intermolecular contacts between 14-3-3 and the S373 motif, emphasizing the phosphorylation dependence of this interaction. Furthermore, the ability of the wild-type or the S244A GST-Cx43 C-terminal fusion protein, but not the S373A fusion protein, to interact with either 14-3-3 or 14-3-3zeta in GST pull-down experiments clearly demonstrated that the S373 motif mediates the direct interaction between Cx43 and 14-3-3 proteins. Blocking growth factor-induced Akt activation and presumably any Akt-mediated phosphorylation of the S373 motif in ROSE 199 cells did not prevent the down-regulation of Cx43-mediated cell-cell communication, suggesting that an Akt-mediated interaction with 14-3-3 was not involved in the disruption of Cx43 function."xsd:string |
| http://purl.uniprot.org/citations/17008717 | http://purl.org/dc/terms/identifier | "doi:10.1110/ps.062172506"xsd:string |
| http://purl.uniprot.org/citations/17008717 | http://purl.uniprot.org/core/author | "Freitas T.A."xsd:string |
| http://purl.uniprot.org/citations/17008717 | http://purl.uniprot.org/core/author | "Park D.J."xsd:string |
| http://purl.uniprot.org/citations/17008717 | http://purl.uniprot.org/core/author | "Wallick C.J."xsd:string |
| http://purl.uniprot.org/citations/17008717 | http://purl.uniprot.org/core/author | "Warn-Cramer B.J."xsd:string |
| http://purl.uniprot.org/citations/17008717 | http://purl.uniprot.org/core/author | "Guyette C.V."xsd:string |
| http://purl.uniprot.org/citations/17008717 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/17008717 | http://purl.uniprot.org/core/name | "Protein Sci"xsd:string |
| http://purl.uniprot.org/citations/17008717 | http://purl.uniprot.org/core/pages | "2344-2355"xsd:string |
| http://purl.uniprot.org/citations/17008717 | http://purl.uniprot.org/core/title | "Molecular dynamics and in vitro analysis of Connexin43: A new 14-3-3 mode-1 interacting protein."xsd:string |
| http://purl.uniprot.org/citations/17008717 | http://purl.uniprot.org/core/volume | "15"xsd:string |
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