| http://purl.uniprot.org/citations/17020884 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17020884 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17020884 | http://www.w3.org/2000/01/rdf-schema#comment | "Recoverin, a member of the neuronal calcium sensor branch of the EF-hand superfamily, serves as a calcium sensor that regulates rhodopsin kinase (RK) activity in retinal rod cells. We report here the NMR structure of Ca(2+)-bound recoverin bound to a functional N-terminal fragment of rhodopsin kinase (residues 1-25, called RK25). The overall main-chain structure of recoverin in the complex is similar to structures of Ca(2+)-bound recoverin in the absence of target (<1.8A root-mean-square deviation). The first eight residues of recoverin at the N terminus are solvent-exposed, enabling the N-terminal myristoyl group to interact with target membranes, and Ca(2+) is bound at the second and third EF-hands of the protein. RK25 in the complex forms an amphipathic helix (residues 4-16). The hydrophobic face of the RK25 helix (Val-9, Val-10, Ala-11, Ala-14, and Phe-15) interacts with an exposed hydrophobic groove on the surface of recoverin lined by side-chain atoms of Trp-31, Phe-35, Phe-49, Ile-52, Tyr-53, Phe-56, Phe-57, Tyr-86, and Leu-90. Residues of recoverin that contact RK25 are highly conserved, suggesting a similar target binding site structure in all neuronal calcium sensor proteins. Site-specific mutagenesis and deletion analysis confirm that the hydrophobic residues at the interface are necessary and sufficient for binding. The recoverin-RK25 complex exhibits Ca(2+)-induced binding to rhodopsin immobilized on concanavalin-A resin. We propose that Ca(2+)-bound recoverin is bound between rhodopsin and RK in a ternary complex on rod outer segment disk membranes, thereby blocking RK interaction with rhodopsin at high Ca(2+)."xsd:string |
| http://purl.uniprot.org/citations/17020884 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m606913200"xsd:string |
| http://purl.uniprot.org/citations/17020884 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m606913200"xsd:string |
| http://purl.uniprot.org/citations/17020884 | http://purl.uniprot.org/core/author | "Levay K."xsd:string |
| http://purl.uniprot.org/citations/17020884 | http://purl.uniprot.org/core/author | "Levay K."xsd:string |
| http://purl.uniprot.org/citations/17020884 | http://purl.uniprot.org/core/author | "Slepak V.Z."xsd:string |
| http://purl.uniprot.org/citations/17020884 | http://purl.uniprot.org/core/author | "Slepak V.Z."xsd:string |
| http://purl.uniprot.org/citations/17020884 | http://purl.uniprot.org/core/author | "Ames J.B."xsd:string |
| http://purl.uniprot.org/citations/17020884 | http://purl.uniprot.org/core/author | "Ames J.B."xsd:string |
| http://purl.uniprot.org/citations/17020884 | http://purl.uniprot.org/core/author | "Wingard J.N."xsd:string |
| http://purl.uniprot.org/citations/17020884 | http://purl.uniprot.org/core/author | "Wingard J.N."xsd:string |
| http://purl.uniprot.org/citations/17020884 | http://purl.uniprot.org/core/author | "Lusin J.D."xsd:string |
| http://purl.uniprot.org/citations/17020884 | http://purl.uniprot.org/core/author | "Lusin J.D."xsd:string |
| http://purl.uniprot.org/citations/17020884 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/17020884 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/17020884 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/17020884 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/17020884 | http://purl.uniprot.org/core/pages | "37237-37245"xsd:string |
| http://purl.uniprot.org/citations/17020884 | http://purl.uniprot.org/core/pages | "37237-37245"xsd:string |
| http://purl.uniprot.org/citations/17020884 | http://purl.uniprot.org/core/title | "Structural basis for calcium-induced inhibition of rhodopsin kinase by recoverin."xsd:string |
| http://purl.uniprot.org/citations/17020884 | http://purl.uniprot.org/core/title | "Structural basis for calcium-induced inhibition of rhodopsin kinase by recoverin."xsd:string |
| http://purl.uniprot.org/citations/17020884 | http://purl.uniprot.org/core/volume | "281"xsd:string |
| http://purl.uniprot.org/citations/17020884 | http://purl.uniprot.org/core/volume | "281"xsd:string |