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http://purl.uniprot.org/citations/17023684http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/17023684http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/17023684http://www.w3.org/2000/01/rdf-schema#comment"Polysialoglycoprotein (PSGP) in salmonid fish egg is a unique glycoprotein bearing alpha2,8-linked polysialic acid (polySia) on its O-linked glycans. Biosynthesis of the polySia chains is developmentally regulated and only occurs at later stage of oogenesis. Two alpha2,8-polysialyltransferases (alpha2,8-polySTs), PST (ST8Sia IV) and STX (ST8Sia II), responsible for the biosynthesis of polySia on N-glycans of glycoproteins, are known in mammals. However, nothing has been known about which alpha2,8-polySTs are involved in the biosynthesis of polySia on O-linked glycans in any glycoproteins. We thus sought to identify cDNA encoding the alpha2,8-polyST involved in polysialylation of PSGP. A clone for PST orthologue, rtPST, and two clones for the STX orthologue, rtSTX-ov and rtSTX-em, were identified in rainbow trout. The deduced amino acid sequence of rtPST shows a high identity (72-77%) to other vertebrate PSTs, while that of rtSTX-ov shows 92% identity with rtSTX-em and a significant identity (63-76%) to other vertebrate STXs. The rtPST exhibited the in vivo alpha2,8-polyST activity, although its in vitro activity was low. However, the rtSTXs showed no in vivo and very low in vitro activities. Interestingly, co-existence of rtPST and rSTX-ov in the reaction mixture synergistically enhanced the alpha2,8-polyST activity. During oogenesis, rtPST was constantly expressed, while the expression of rtSTX-ov was not increased until polySia chain is abundantly biosynthesized in the later stage. rtSTX-em was not expressed in ovary. These results suggest that the enhanced expression of rtSTX-ov under the co-expression with rtPST may be important for the biosynthesis of polySia on O-linked glycans of PSGP."xsd:string
http://purl.uniprot.org/citations/17023684http://purl.org/dc/terms/identifier"doi:10.1093/jb/mvj200"xsd:string
http://purl.uniprot.org/citations/17023684http://purl.org/dc/terms/identifier"doi:10.1093/jb/mvj200"xsd:string
http://purl.uniprot.org/citations/17023684http://purl.uniprot.org/core/author"Sato C."xsd:string
http://purl.uniprot.org/citations/17023684http://purl.uniprot.org/core/author"Sato C."xsd:string
http://purl.uniprot.org/citations/17023684http://purl.uniprot.org/core/author"Matsuda T."xsd:string
http://purl.uniprot.org/citations/17023684http://purl.uniprot.org/core/author"Matsuda T."xsd:string
http://purl.uniprot.org/citations/17023684http://purl.uniprot.org/core/author"Kitajima K."xsd:string
http://purl.uniprot.org/citations/17023684http://purl.uniprot.org/core/author"Kitajima K."xsd:string
http://purl.uniprot.org/citations/17023684http://purl.uniprot.org/core/author"Matsuno M."xsd:string
http://purl.uniprot.org/citations/17023684http://purl.uniprot.org/core/author"Matsuno M."xsd:string
http://purl.uniprot.org/citations/17023684http://purl.uniprot.org/core/author"Asahina S."xsd:string
http://purl.uniprot.org/citations/17023684http://purl.uniprot.org/core/author"Asahina S."xsd:string
http://purl.uniprot.org/citations/17023684http://purl.uniprot.org/core/author"Colley K."xsd:string
http://purl.uniprot.org/citations/17023684http://purl.uniprot.org/core/author"Colley K."xsd:string
http://purl.uniprot.org/citations/17023684http://purl.uniprot.org/core/date"2006"xsd:gYear
http://purl.uniprot.org/citations/17023684http://purl.uniprot.org/core/date"2006"xsd:gYear
http://purl.uniprot.org/citations/17023684http://purl.uniprot.org/core/name"J. Biochem."xsd:string
http://purl.uniprot.org/citations/17023684http://purl.uniprot.org/core/name"J Biochem"xsd:string
http://purl.uniprot.org/citations/17023684http://purl.uniprot.org/core/pages"687-701"xsd:string
http://purl.uniprot.org/citations/17023684http://purl.uniprot.org/core/pages"687-701"xsd:string
http://purl.uniprot.org/citations/17023684http://purl.uniprot.org/core/title"Involvement of the alpha2,8-polysialyltransferases II/STX and IV/PST in the biosynthesis of polysialic acid chains on the O-linked glycoproteins in rainbow trout ovary."xsd:string
http://purl.uniprot.org/citations/17023684http://purl.uniprot.org/core/title"Involvement of the alpha2,8-polysialyltransferases II/STX and IV/PST in the biosynthesis of polysialic acid chains on the O-linked glycoproteins in rainbow trout ovary."xsd:string
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