| http://purl.uniprot.org/citations/17043108 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17043108 | http://www.w3.org/2000/01/rdf-schema#comment | "The transcriptional coactivator p/CIP(SRC-3/AIB1/ACTR/RAC3) binds liganded nuclear hormone receptors and facilitates transcription by directly recruiting accessory factors such as acetyltransferase CBP/p300 and the coactivator arginine methyltransferase CARM1. In the present study, we have established that recombinant p/CIP (p300/CBP interacting protein) is robustly methylated by CARM1 in vitro but not by other protein arginine methyltransferase family members. Metabolic labeling of MCF-7 breast cancer cells with S-adenosyl-L-[methyl-(3)H]methionine and immunoblotting using dimethyl arginine-specific antibodies demonstrated that p/CIP is specifically methylated in intact cells. In addition, methylation of full-length p/CIP is not supported by extracts derived from CARM1(-/-) mouse embryo fibroblasts, indicating that CARM1 is required for p/CIP methylation. Using mass spectrometry, we have identified three CARM1-dependent methylation sites located in a glutamine-rich region within the carboxy terminus of p/CIP which are conserved among all steroid receptor coactivator proteins. These results were confirmed by in vitro methylation of p/CIP using carboxy-terminal truncation mutants and synthetic peptides as substrates for CARM1. Analysis of methylation site mutants revealed that arginine methylation causes an increase in full-length p/CIP turnover as a result of enhanced degradation. Additionally, methylation negatively impacts transcription via a second mechanism by impairing the ability of p/CIP to associate with CBP. Collectively, our data highlight coactivator methylation as an important regulatory mechanism in hormonal signaling."xsd:string |
| http://purl.uniprot.org/citations/17043108 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.00815-06"xsd:string |
| http://purl.uniprot.org/citations/17043108 | http://purl.uniprot.org/core/author | "Bedford M.T."xsd:string |
| http://purl.uniprot.org/citations/17043108 | http://purl.uniprot.org/core/author | "Zhao Q."xsd:string |
| http://purl.uniprot.org/citations/17043108 | http://purl.uniprot.org/core/author | "Cheng D."xsd:string |
| http://purl.uniprot.org/citations/17043108 | http://purl.uniprot.org/core/author | "Torchia J."xsd:string |
| http://purl.uniprot.org/citations/17043108 | http://purl.uniprot.org/core/author | "Tini M."xsd:string |
| http://purl.uniprot.org/citations/17043108 | http://purl.uniprot.org/core/author | "Underhill C."xsd:string |
| http://purl.uniprot.org/citations/17043108 | http://purl.uniprot.org/core/author | "Naeem H."xsd:string |
| http://purl.uniprot.org/citations/17043108 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17043108 | http://purl.uniprot.org/core/name | "Mol Cell Biol"xsd:string |
| http://purl.uniprot.org/citations/17043108 | http://purl.uniprot.org/core/pages | "120-134"xsd:string |
| http://purl.uniprot.org/citations/17043108 | http://purl.uniprot.org/core/title | "The activity and stability of the transcriptional coactivator p/CIP/SRC-3 are regulated by CARM1-dependent methylation."xsd:string |
| http://purl.uniprot.org/citations/17043108 | http://purl.uniprot.org/core/volume | "27"xsd:string |
| http://purl.uniprot.org/citations/17043108 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17043108 |
| http://purl.uniprot.org/citations/17043108 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17043108 |
| http://purl.uniprot.org/uniprot/#_A0A0U1RP23-mappedCitation-17043108 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17043108 |
| http://purl.uniprot.org/uniprot/#_A0A0U1RP46-mappedCitation-17043108 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17043108 |
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| http://purl.uniprot.org/uniprot/#_A0A0U1RPQ3-mappedCitation-17043108 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17043108 |
| http://purl.uniprot.org/uniprot/#_A0A1L1ST60-mappedCitation-17043108 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17043108 |
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| http://purl.uniprot.org/uniprot/#_A5D6Q2-mappedCitation-17043108 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17043108 |
| http://purl.uniprot.org/uniprot/#_A0A0U1RQB6-mappedCitation-17043108 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17043108 |