| http://purl.uniprot.org/citations/17053064 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17053064 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17053064 | http://www.w3.org/2000/01/rdf-schema#comment | "RNase P, which catalyzes the magnesium-dependent 5'-end maturation of tRNAs in all three domains of life, is composed of one essential RNA and a varying number of protein subunits depending on the source: at least one in bacteria, four in archaea, and nine in eukarya. To address why multiple protein subunits are needed for archaeal/eukaryal RNase P catalysis, in contrast to their bacterial relative, in vitro reconstitution of these holoenzymes is a prerequisite. Using recombinant subunits, we have reconstituted in vitro the RNase P holoenzyme from the thermophilic archaeon Pyrococcus furiosus (Pfu) and furthered our understanding regarding its functional organization and assembly pathway(s). Whereas Pfu RNase P RNA (RPR) alone is capable of multiple turnover, addition of all four RNase P protein (Rpp) subunits to Pfu RPR results in a 25-fold increase in its k(cat) and a 170-fold decrease in K(m). In fact, even in the presence of only one of two specific pairs of Rpps, the RPR displays activity at lower substrate and magnesium concentrations. Moreover, a pared-down, mini-Pfu RNase P was identified with an RPR deletion mutant. Results from our kinetic and footprinting studies on Pfu RNase P, together with insights from recent structures of bacterial RPRs, provide a framework for appreciating the role of multiple Rpps in archaeal RNase P."xsd:string |
| http://purl.uniprot.org/citations/17053064 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.0608000103"xsd:string |
| http://purl.uniprot.org/citations/17053064 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.0608000103"xsd:string |
| http://purl.uniprot.org/citations/17053064 | http://purl.uniprot.org/core/author | "Gopalan V."xsd:string |
| http://purl.uniprot.org/citations/17053064 | http://purl.uniprot.org/core/author | "Gopalan V."xsd:string |
| http://purl.uniprot.org/citations/17053064 | http://purl.uniprot.org/core/author | "Pulukkunat D.K."xsd:string |
| http://purl.uniprot.org/citations/17053064 | http://purl.uniprot.org/core/author | "Pulukkunat D.K."xsd:string |
| http://purl.uniprot.org/citations/17053064 | http://purl.uniprot.org/core/author | "Tsai H.Y."xsd:string |
| http://purl.uniprot.org/citations/17053064 | http://purl.uniprot.org/core/author | "Tsai H.Y."xsd:string |
| http://purl.uniprot.org/citations/17053064 | http://purl.uniprot.org/core/author | "Woznick W.K."xsd:string |
| http://purl.uniprot.org/citations/17053064 | http://purl.uniprot.org/core/author | "Woznick W.K."xsd:string |
| http://purl.uniprot.org/citations/17053064 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/17053064 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/17053064 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/17053064 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/17053064 | http://purl.uniprot.org/core/pages | "16147-16152"xsd:string |
| http://purl.uniprot.org/citations/17053064 | http://purl.uniprot.org/core/pages | "16147-16152"xsd:string |
| http://purl.uniprot.org/citations/17053064 | http://purl.uniprot.org/core/title | "Functional reconstitution and characterization of Pyrococcus furiosus RNase P."xsd:string |
| http://purl.uniprot.org/citations/17053064 | http://purl.uniprot.org/core/title | "Functional reconstitution and characterization of Pyrococcus furiosus RNase P."xsd:string |
| http://purl.uniprot.org/citations/17053064 | http://purl.uniprot.org/core/volume | "103"xsd:string |
| http://purl.uniprot.org/citations/17053064 | http://purl.uniprot.org/core/volume | "103"xsd:string |
| http://purl.uniprot.org/citations/17053064 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17053064 |
| http://purl.uniprot.org/citations/17053064 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17053064 |