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Background

Embryonic patterning mechanisms regulate the cytoskeletal machinery that drives morphogenesis, but there are few cases where links between patterning mechanisms and morphogenesis are well understood. We have used a combination of genetics, in vivo imaging, and cell manipulations to identify such links in C. elegans gastrulation. Gastrulation in C. elegans begins with the internalization of endodermal precursor cells in a process that depends on apical constriction of ingressing cells.

Results

We show that ingression of the endodermal precursor cells is regulated by pathways, including a Wnt-Frizzled signaling pathway, that specify endodermal cell fate. We find that Wnt signaling has a role in gastrulation in addition to its earlier roles in regulating endodermal cell fate and cell-cycle timing. In the absence of Wnt signaling, endodermal precursor cells polarize and enrich myosin II apically but fail to contract their apical surfaces. We show that a regulatory myosin light chain normally becomes phosphorylated on the apical side of ingressing cells at a conserved site that can lead to myosin-filament formation and contraction of actomyosin networks and that this phosphorylation depends on Wnt signaling.

Conclusions

We conclude that Wnt signaling regulates C. elegans gastrulation through regulatory myosin light-chain phosphorylation, which results in the contraction of the apical surface of ingressing cells. These findings forge new links between cell-fate specification and morphogenesis, and they represent a novel mechanism by which Wnt signaling can regulate morphogenesis."xsd:string
http://purl.uniprot.org/citations/17055977http://purl.org/dc/terms/identifier"doi:10.1016/j.cub.2006.08.090"xsd:string
http://purl.uniprot.org/citations/17055977http://purl.uniprot.org/core/author"Lee J.Y."xsd:string
http://purl.uniprot.org/citations/17055977http://purl.uniprot.org/core/author"Hardin J."xsd:string
http://purl.uniprot.org/citations/17055977http://purl.uniprot.org/core/author"Goldstein B."xsd:string
http://purl.uniprot.org/citations/17055977http://purl.uniprot.org/core/author"Marston D.J."xsd:string
http://purl.uniprot.org/citations/17055977http://purl.uniprot.org/core/author"Walston T."xsd:string
http://purl.uniprot.org/citations/17055977http://purl.uniprot.org/core/author"Halberstadt A."xsd:string
http://purl.uniprot.org/citations/17055977http://purl.uniprot.org/core/date"2006"xsd:gYear
http://purl.uniprot.org/citations/17055977http://purl.uniprot.org/core/name"Curr Biol"xsd:string
http://purl.uniprot.org/citations/17055977http://purl.uniprot.org/core/pages"1986-1997"xsd:string
http://purl.uniprot.org/citations/17055977http://purl.uniprot.org/core/title"Wnt/Frizzled signaling controls C. elegans gastrulation by activating actomyosin contractility."xsd:string
http://purl.uniprot.org/citations/17055977http://purl.uniprot.org/core/volume"16"xsd:string
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