| http://purl.uniprot.org/citations/17056592 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17056592 | http://www.w3.org/2000/01/rdf-schema#comment | "Calpains are Ca(2+)-dependent cysteine proteases known to be important for the regulation of cell functions and which aberrant activation causes cell death in a number of degenerative disorders. To provide a tool for monitoring the status of calpain activity in vivo under physiological and pathological conditions, we created a mouse model that expresses ubiquitously a fluorescent reporter consisting of eCFP and eYFP separated by a linker cleavable by the ubiquitous calpains. We named this mouse CAFI for calpain activity monitored by FRET imaging. Our validation studies demonstrated that the level of calpain activity correlates with a decrease in FRET (fluorescence resonance energy transfer) between the two fluorescent proteins. Using this model, we observed a small level of activity after denervation and fasting, a high level of activity during muscle regeneration and ischemia, and local activity in damaged myofibers after exercise. Finally, we crossed the CAFI mouse with the alpha-sarcoglycan-deficient model, demonstrating an increase of calpain activity at the steady state. Altogether, our results present evidence that CAFI mice could be a valuable tool in which to follow calpain activity at physiological levels and in disease states."xsd:string |
| http://purl.uniprot.org/citations/17056592 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m608803200"xsd:string |
| http://purl.uniprot.org/citations/17056592 | http://purl.uniprot.org/core/author | "Han Y."xsd:string |
| http://purl.uniprot.org/citations/17056592 | http://purl.uniprot.org/core/author | "Richard I."xsd:string |
| http://purl.uniprot.org/citations/17056592 | http://purl.uniprot.org/core/author | "Bourg N."xsd:string |
| http://purl.uniprot.org/citations/17056592 | http://purl.uniprot.org/core/author | "Raynaud F."xsd:string |
| http://purl.uniprot.org/citations/17056592 | http://purl.uniprot.org/core/author | "Bartoli M."xsd:string |
| http://purl.uniprot.org/citations/17056592 | http://purl.uniprot.org/core/author | "Stockholm D."xsd:string |
| http://purl.uniprot.org/citations/17056592 | http://purl.uniprot.org/core/author | "Borel P."xsd:string |
| http://purl.uniprot.org/citations/17056592 | http://purl.uniprot.org/core/author | "Armande N."xsd:string |
| http://purl.uniprot.org/citations/17056592 | http://purl.uniprot.org/core/author | "Delevacque A."xsd:string |
| http://purl.uniprot.org/citations/17056592 | http://purl.uniprot.org/core/author | "Seddik K."xsd:string |
| http://purl.uniprot.org/citations/17056592 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/17056592 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/17056592 | http://purl.uniprot.org/core/pages | "39672-39680"xsd:string |
| http://purl.uniprot.org/citations/17056592 | http://purl.uniprot.org/core/title | "A mouse model for monitoring calpain activity under physiological and pathological conditions."xsd:string |
| http://purl.uniprot.org/citations/17056592 | http://purl.uniprot.org/core/volume | "281"xsd:string |
| http://purl.uniprot.org/citations/17056592 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17056592 |
| http://purl.uniprot.org/citations/17056592 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17056592 |
| http://purl.uniprot.org/uniprot/#_P00493-mappedCitation-17056592 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17056592 |
| http://purl.uniprot.org/uniprot/#_Q5SWB0-mappedCitation-17056592 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17056592 |
| http://purl.uniprot.org/uniprot/#_Q5SWB4-mappedCitation-17056592 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17056592 |
| http://purl.uniprot.org/uniprot/#_P82350-mappedCitation-17056592 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17056592 |
| http://purl.uniprot.org/uniprot/#_Q99KF5-mappedCitation-17056592 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17056592 |