| http://purl.uniprot.org/citations/17065558 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17065558 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17065558 | http://www.w3.org/2000/01/rdf-schema#comment | "Chromatin assembly factor-1 (CAF-1), a complex consisting of p150, p60, and p48 subunits, is highly conserved from yeast to humans and facilitates nucleosome assembly of newly replicated DNA in vitro. To investigate roles of CAF-1 in vertebrates, we generated two conditional DT40 mutants, respectively, devoid of CAF-1p150 and p60. Depletion of each of these CAF-1 subunits led to delayed S-phase progression concomitant with slow DNA synthesis, followed by accumulation in late S/G2 phase and aberrant mitosis associated with extra centrosomes, and then the final consequence was cell death. We demonstrated that CAF-1 is necessary for rapid nucleosome formation during DNA replication in vivo as well as in vitro. Loss of CAF-1 was not associated with the apparent induction of phosphorylations of S-checkpoint kinases Chk1 and Chk2. To elucidate the precise role of domain(s) in CAF-1p150, functional dissection analyses including rescue assays were preformed. Results showed that the binding abilities of CAF-1p150 with CAF-1p60 and DNA polymerase sliding clamp proliferating cell nuclear antigen (PCNA) but not with heterochromatin protein HP1-gamma are required for cell viability. These observations highlighted the essential role of CAF-1-dependent nucleosome assembly in DNA replication and cell proliferation through its interaction with PCNA."xsd:string |
| http://purl.uniprot.org/citations/17065558 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.e06-05-0426"xsd:string |
| http://purl.uniprot.org/citations/17065558 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.e06-05-0426"xsd:string |
| http://purl.uniprot.org/citations/17065558 | http://purl.uniprot.org/core/author | "Nakayama T."xsd:string |
| http://purl.uniprot.org/citations/17065558 | http://purl.uniprot.org/core/author | "Nakayama T."xsd:string |
| http://purl.uniprot.org/citations/17065558 | http://purl.uniprot.org/core/author | "Ono T."xsd:string |
| http://purl.uniprot.org/citations/17065558 | http://purl.uniprot.org/core/author | "Ono T."xsd:string |
| http://purl.uniprot.org/citations/17065558 | http://purl.uniprot.org/core/author | "Shibahara K."xsd:string |
| http://purl.uniprot.org/citations/17065558 | http://purl.uniprot.org/core/author | "Shibahara K."xsd:string |
| http://purl.uniprot.org/citations/17065558 | http://purl.uniprot.org/core/author | "Fukagawa T."xsd:string |
| http://purl.uniprot.org/citations/17065558 | http://purl.uniprot.org/core/author | "Fukagawa T."xsd:string |
| http://purl.uniprot.org/citations/17065558 | http://purl.uniprot.org/core/author | "Takami Y."xsd:string |
| http://purl.uniprot.org/citations/17065558 | http://purl.uniprot.org/core/author | "Takami Y."xsd:string |
| http://purl.uniprot.org/citations/17065558 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17065558 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17065558 | http://purl.uniprot.org/core/name | "Mol. Biol. Cell"xsd:string |
| http://purl.uniprot.org/citations/17065558 | http://purl.uniprot.org/core/name | "Mol. Biol. Cell"xsd:string |
| http://purl.uniprot.org/citations/17065558 | http://purl.uniprot.org/core/pages | "129-141"xsd:string |
| http://purl.uniprot.org/citations/17065558 | http://purl.uniprot.org/core/pages | "129-141"xsd:string |
| http://purl.uniprot.org/citations/17065558 | http://purl.uniprot.org/core/title | "Essential role of chromatin assembly factor-1-mediated rapid nucleosome assembly for DNA replication and cell division in vertebrate cells."xsd:string |
| http://purl.uniprot.org/citations/17065558 | http://purl.uniprot.org/core/title | "Essential role of chromatin assembly factor-1-mediated rapid nucleosome assembly for DNA replication and cell division in vertebrate cells."xsd:string |
| http://purl.uniprot.org/citations/17065558 | http://purl.uniprot.org/core/volume | "18"xsd:string |
| http://purl.uniprot.org/citations/17065558 | http://purl.uniprot.org/core/volume | "18"xsd:string |