| http://purl.uniprot.org/citations/17070676 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17070676 | http://www.w3.org/2000/01/rdf-schema#comment | "The transcription factor, Vnd, is a dual regulator that specifies ventral neuroblast identity in Drosophila by both repressing and activating target genes. Vnd and its homologues have a conserved amino acid sequence, the Nk-2 box or Nk specific domain, as well a conserved DNA-binding homeodomain and an EhI-type Groucho interaction domain. However, the function of the conserved Nk-2 box has not been fully defined. To explore its function, we deleted the Nk-2 box and compared the regulatory activity of mutant Vnd in transgenic over-expression assays to that of the wild-type protein. We were unable to assign regulatory activity to the Nk-2 box using an over-expression assay, because the mutant protein activated expression of endogenous Vnd, masking a requirement for the Nk-2 box. However, in transgenic rescue assays, Vnd lacking the Nk-2 box repressed ind expression at 30% lower levels than the wild-type protein. Moreover, in transient transfection assays using Gal4 DNA-binding domain-Vnd chimeras, the repression activity of Vnd lacking the Nk-2 box was compromised. Because Vnd represses target gene expression in conjunction with Groucho, we asked whether the Nk-2 box affects Vnd's ability to interact with this co-repressor. Vnd lacking the Nk-2 box binds Groucho 30% less efficiently than wild-type Vnd in co-immunoprecipitations. These data suggest that the Nk-2 box contributes to the repression activity of Vnd by stabilizing its interaction with the co-repressor, Groucho."xsd:string |
| http://purl.uniprot.org/citations/17070676 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.mod.2006.07.009"xsd:string |
| http://purl.uniprot.org/citations/17070676 | http://purl.uniprot.org/core/author | "Zhang H."xsd:string |
| http://purl.uniprot.org/citations/17070676 | http://purl.uniprot.org/core/author | "Mellerick D.M."xsd:string |
| http://purl.uniprot.org/citations/17070676 | http://purl.uniprot.org/core/author | "Uhler J."xsd:string |
| http://purl.uniprot.org/citations/17070676 | http://purl.uniprot.org/core/author | "Syu L.J."xsd:string |
| http://purl.uniprot.org/citations/17070676 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17070676 | http://purl.uniprot.org/core/name | "Mech Dev"xsd:string |
| http://purl.uniprot.org/citations/17070676 | http://purl.uniprot.org/core/pages | "1-10"xsd:string |
| http://purl.uniprot.org/citations/17070676 | http://purl.uniprot.org/core/title | "The Nk-2 box of the Drosophila homeodomain protein, Vnd, contributes to its repression activity in a Groucho-dependent manner."xsd:string |
| http://purl.uniprot.org/citations/17070676 | http://purl.uniprot.org/core/volume | "124"xsd:string |
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| http://purl.uniprot.org/uniprot/#_P10083-mappedCitation-17070676 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17070676 |
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| http://purl.uniprot.org/uniprot/#_Q0KHX8-mappedCitation-17070676 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17070676 |