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http://purl.uniprot.org/citations/17074191http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/17074191http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To investigate the expression of the T cell-specific transcription factors T-bet/GATA-3 in recurrent aphthous ulcerations (RAU).

Methods

Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation from RAU patients and healthy controls. Expressions of T-bet and GATA-3 mRNA and protein in the PBMCs were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot respectively. The concentrations of gamma-interferon (gamma-IFN) and interleukin-4 (IL-4) in the serum were determined by enzyme-linked immunosorbent assay (ELISA).

Results

The expressions of T-bet mRNA were 0.73 +/-0.14 in the control group and 0.12 +/-0.04 in the recurrent aphthous ulcerations group, the levels of T-bet protein expressions were 0.73 +/- 0.18 in the control group and 0.24 +/-0.09 in the recurrent aphthous ulcerations group, there was a significant difference between the two groups (P < 0.01). The levels of GATA-3 mRNA expressions were 0.89 +/- 0.11 in the control group and 1.30 +/-0.13 in the recurrent aphthous ulcerations group; GATA-3 protein expressions were 1.09 +/- 0.16 in the control group and 1.80 +/-0.16 in the recurrent aphthous ulcerations group, there was a significant difference between the two groups (P < 0.01). In the control group, the concentration of gamma-interferon in the serum was (22.15 +/-1.52) ng/L, which was significantly elevated compared with that in the recurrent aphthous ulcerations group (16.32 +/-0.22) ng/L (P < 0.01), in the control group, the concentration of IL-4 in the serum was (25.58 +/-2.59) ng/L, which was significantly lower than that of the recurrent aphthous ulcerations group (43.60 +/-3.15) ng/L (P < 0.01). The ratio of gamma-IFN and IL-4 was positively correlated with the ratio of T-bet and GATA-3 mRNA and protein expression (r = 0.96, 0.94, P < 0.01).

Conclusions

Imbalance of transcription factors T-bet and GATA-3 may be one of the key factors in immune dysregulation of recurrent aphthous ulcerations."xsd:string
http://purl.uniprot.org/citations/17074191http://purl.uniprot.org/core/author"Yao Y."xsd:string
http://purl.uniprot.org/citations/17074191http://purl.uniprot.org/core/author"Li L.H."xsd:string
http://purl.uniprot.org/citations/17074191http://purl.uniprot.org/core/author"Zhu J.X."xsd:string
http://purl.uniprot.org/citations/17074191http://purl.uniprot.org/core/author"Huang Z.H."xsd:string
http://purl.uniprot.org/citations/17074191http://purl.uniprot.org/core/author"Liu A.G."xsd:string
http://purl.uniprot.org/citations/17074191http://purl.uniprot.org/core/author"Shan L.Y."xsd:string
http://purl.uniprot.org/citations/17074191http://purl.uniprot.org/core/date"2006"xsd:gYear
http://purl.uniprot.org/citations/17074191http://purl.uniprot.org/core/name"Zhonghua Kou Qiang Yi Xue Za Zhi"xsd:string
http://purl.uniprot.org/citations/17074191http://purl.uniprot.org/core/pages"494-497"xsd:string
http://purl.uniprot.org/citations/17074191http://purl.uniprot.org/core/title"[Expression of T cell-specific transcription factors T-bet and GATA-3 in recurrent aphthous ulcerations]."xsd:string
http://purl.uniprot.org/citations/17074191http://purl.uniprot.org/core/volume"41"xsd:string
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