| http://purl.uniprot.org/citations/17075075 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17075075 | http://www.w3.org/2000/01/rdf-schema#comment | "Cadmium is a major environmental pollutant that enters human food via accumulation in crop plants. Responses of plants to cadmium exposure--which directly influence accumulation rates--are not well understood. In general, little is known about stress-elicited changes in plants at the proteome level. Alterations in the root proteome of hydroponically grown Arabidopsis thaliana plants treated with 10 microM Cd(2+) for 24 h are reported here. These conditions trigger the synthesis of phytochelatins (PCs), glutathione-derived metal-binding peptides, shown here as PC2 accumulation. Two-dimensional gel electrophoresis using different pH gradients in the first dimension detected on average approximately 1100 spots per gel type. Forty-one spots indicated significant changes in protein abundance upon Cd(2+) treatment. Seventeen proteins found in 25 spots were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Selected results were independently confirmed by western analysis and selective enrichment of a protein family (glutathione S-transferases) through affinity chromatography. Most of the identified proteins belong to four different classes: metabolic enzymes such as ATP sulphurylase, glycine hydroxymethyltransferase, and trehalose-6-phosphate phosphatase; glutathione S-transferases; latex allergen-like proteins; and unknown proteins. These results represent a basis for reverse genetics studies to better understand plant responses to toxic metal exposure and to the generation of internal sinks for reduced sulphur."xsd:string |
| http://purl.uniprot.org/citations/17075075 | http://purl.org/dc/terms/identifier | "doi:10.1093/jxb/erl170"xsd:string |
| http://purl.uniprot.org/citations/17075075 | http://purl.uniprot.org/core/author | "Clemens S."xsd:string |
| http://purl.uniprot.org/citations/17075075 | http://purl.uniprot.org/core/author | "von Roepenack-Lahaye E."xsd:string |
| http://purl.uniprot.org/citations/17075075 | http://purl.uniprot.org/core/author | "Roth U."xsd:string |
| http://purl.uniprot.org/citations/17075075 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/17075075 | http://purl.uniprot.org/core/name | "J Exp Bot"xsd:string |
| http://purl.uniprot.org/citations/17075075 | http://purl.uniprot.org/core/pages | "4003-4013"xsd:string |
| http://purl.uniprot.org/citations/17075075 | http://purl.uniprot.org/core/title | "Proteome changes in Arabidopsis thaliana roots upon exposure to Cd2+."xsd:string |
| http://purl.uniprot.org/citations/17075075 | http://purl.uniprot.org/core/volume | "57"xsd:string |
| http://purl.uniprot.org/citations/17075075 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17075075 |
| http://purl.uniprot.org/citations/17075075 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17075075 |
| http://purl.uniprot.org/uniprot/F4I7I0#attribution-7BB80712CC3968CFE0401F58B632487B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/17075075 |
| http://purl.uniprot.org/uniprot/Q9LIK9#attribution-7BB80712CC3968CFE0401F58B632487B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/17075075 |
| http://purl.uniprot.org/uniprot/Q9FX54#attribution-7BB80712CC3968CFE0401F58B632487B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/17075075 |
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| http://purl.uniprot.org/uniprot/P42760#attribution-7BB80712CC3968CFE0401F58B632487B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/17075075 |
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| http://purl.uniprot.org/uniprot/Q5HZ05#attribution-7BB80712CC3968CFE0401F58B632487B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/17075075 |