| http://purl.uniprot.org/citations/17088291 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17088291 | http://www.w3.org/2000/01/rdf-schema#comment | "Nonsense-mediated mRNA decay (NMD) is a eukaryotic quality control mechanism that identifies and eliminates aberrant mRNAs containing a premature termination codon (PTC). Although, key trans-acting NMD factors, UPF1, UPF2 and UPF3 are conserved in yeast and mammals, the cis-acting NMD elements are different. In yeast, short specific sequences or long 3'-untranslated regions (3'-UTRs) render an mRNA subject to NMD, while in mammals' 3'-UTR located introns trigger NMD. Plants also possess an NMD system, although little is known about how it functions. We have elaborated an agroinfiltration-based transient NMD assay system and defined the cis-acting elements that mediate plant NMD. We show that unusually long 3'-UTRs or the presence of introns in the 3'-UTR can subject mRNAs to NMD. These data suggest that both long 3'-UTR-based and intron-based PTC definition operated in the common ancestors of extant eukaryotes (stem eukaryotes) and support the theory that intron-based NMD facilitated the spreading of introns in stem eukaryotes. We have also identified plant UPF1 and showed that tethering of UPF1 to either the 5'- or 3'-UTR of an mRNA results in reduced transcript accumulation. Thus, plant UPF1 might bind to mRNA in a late, irreversible phase of NMD."xsd:string |
| http://purl.uniprot.org/citations/17088291 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gkl737"xsd:string |
| http://purl.uniprot.org/citations/17088291 | http://purl.uniprot.org/core/author | "Barta E."xsd:string |
| http://purl.uniprot.org/citations/17088291 | http://purl.uniprot.org/core/author | "Kerenyi Z."xsd:string |
| http://purl.uniprot.org/citations/17088291 | http://purl.uniprot.org/core/author | "Merai Z."xsd:string |
| http://purl.uniprot.org/citations/17088291 | http://purl.uniprot.org/core/author | "Silhavy D."xsd:string |
| http://purl.uniprot.org/citations/17088291 | http://purl.uniprot.org/core/author | "Bartos I."xsd:string |
| http://purl.uniprot.org/citations/17088291 | http://purl.uniprot.org/core/author | "Kertesz S."xsd:string |
| http://purl.uniprot.org/citations/17088291 | http://purl.uniprot.org/core/author | "Palfy T."xsd:string |
| http://purl.uniprot.org/citations/17088291 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/17088291 | http://purl.uniprot.org/core/name | "Nucleic Acids Res"xsd:string |
| http://purl.uniprot.org/citations/17088291 | http://purl.uniprot.org/core/pages | "6147-6157"xsd:string |
| http://purl.uniprot.org/citations/17088291 | http://purl.uniprot.org/core/title | "Both introns and long 3'-UTRs operate as cis-acting elements to trigger nonsense-mediated decay in plants."xsd:string |
| http://purl.uniprot.org/citations/17088291 | http://purl.uniprot.org/core/volume | "34"xsd:string |
| http://purl.uniprot.org/citations/17088291 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17088291 |
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| http://purl.uniprot.org/uniprot/#_Q9FJR0-mappedCitation-17088291 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17088291 |
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| http://purl.uniprot.org/uniprot/Q9FVW4 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/17088291 |