| http://purl.uniprot.org/citations/1709810 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1709810 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1709810 | http://www.w3.org/2000/01/rdf-schema#comment | "The amino acid sequence of human alpha-fetoprotein, a 67-kDa protein present in mammalian embryonic serum, was verified by fast atom bombardment mass spectrometric (FAB/MS) analyses of three different enzymatic digests of the protein. Human alpha-fetoprotein obtained from a large-scale cell culture was digested with trypsin and V-8 protease either separately on two different samples or combined on the same one. The V-8 protease digest of the protein was partially fractionated by HPLC; the other samples were directly analyzed by FAB/MS without previous purification steps. About 90% of the alpha-fetoprotein amino acid sequence was verified by mass spectrometric analysis; this also confirmed that the cell-derived protein is identical with the hepatoma-derived protein. FAB analysis revealed that the N terminus of the mature protein is arginine rather than threonine, with the threonine occupying the second position. Therefore, the processing site of the alpha-fetoprotein signal peptide during maturation of the protein occurs at the N-terminal side of the arginine residue formerly indicated as residue-1. Thus mature alpha-fetoprotein contains 591 amino acids rather than 590."xsd:string |
| http://purl.uniprot.org/citations/1709810 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi00234a032"xsd:string |
| http://purl.uniprot.org/citations/1709810 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi00234a032"xsd:string |
| http://purl.uniprot.org/citations/1709810 | http://purl.uniprot.org/core/author | "Marino G."xsd:string |
| http://purl.uniprot.org/citations/1709810 | http://purl.uniprot.org/core/author | "Marino G."xsd:string |
| http://purl.uniprot.org/citations/1709810 | http://purl.uniprot.org/core/author | "Pucci P."xsd:string |
| http://purl.uniprot.org/citations/1709810 | http://purl.uniprot.org/core/author | "Pucci P."xsd:string |
| http://purl.uniprot.org/citations/1709810 | http://purl.uniprot.org/core/author | "Malorni A."xsd:string |
| http://purl.uniprot.org/citations/1709810 | http://purl.uniprot.org/core/author | "Malorni A."xsd:string |
| http://purl.uniprot.org/citations/1709810 | http://purl.uniprot.org/core/author | "Tecce M.F."xsd:string |
| http://purl.uniprot.org/citations/1709810 | http://purl.uniprot.org/core/author | "Tecce M.F."xsd:string |
| http://purl.uniprot.org/citations/1709810 | http://purl.uniprot.org/core/author | "Siciliano R."xsd:string |
| http://purl.uniprot.org/citations/1709810 | http://purl.uniprot.org/core/author | "Siciliano R."xsd:string |
| http://purl.uniprot.org/citations/1709810 | http://purl.uniprot.org/core/author | "Ceccarini C."xsd:string |
| http://purl.uniprot.org/citations/1709810 | http://purl.uniprot.org/core/author | "Ceccarini C."xsd:string |
| http://purl.uniprot.org/citations/1709810 | http://purl.uniprot.org/core/author | "Terrana B."xsd:string |
| http://purl.uniprot.org/citations/1709810 | http://purl.uniprot.org/core/author | "Terrana B."xsd:string |
| http://purl.uniprot.org/citations/1709810 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/1709810 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/1709810 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/1709810 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/1709810 | http://purl.uniprot.org/core/pages | "5061-5066"xsd:string |
| http://purl.uniprot.org/citations/1709810 | http://purl.uniprot.org/core/pages | "5061-5066"xsd:string |