| http://purl.uniprot.org/citations/17099216 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17099216 | http://www.w3.org/2000/01/rdf-schema#comment | "Elastic fibers are complex structures composed of a tropoelastin inner core and microfibril outer mantle guiding tropoelastin deposition. Microfibrillar proteins mainly include fibrillins and microfibril-associated glycoproteins (MAGPs). MAGP-2 exhibits developmental expression peaking at elastic fiber onset, suggesting that MAGP-2 mediates elastic fiber assembly. To determine whether MAGP-2 regulates elastic fiber assembly, we used an in vitro model featuring doxycycline-regulated cells conditionally overexpressing exogenous MAGP-2 and constitutively expressing enhanced green fluorescent protein-tagged tropoelastin. Analysis by immunofluorescent staining showed that MAGP-2 overexpression dramatically increased elastic fibers levels, independently of extracellular levels of soluble tropoelastin, indicating that MAGP-2 stimulates elastic fiber assembly. This was associated with increased levels of matrix-associated MAGP-2. Electron microscopy showed that MAGP-2 specifically associates with microfibrils and that elastin globules primarily colocalize with MAGP-2-associated microfibrils, suggesting that microfibril-associated MAGP-2 facilitates elastic fiber assembly. MAGP-2 overexpression did not change levels of matrix-associated fibrillin-1, MAGP-1, fibulin-2, fibulin-5, or emilin-1, suggesting that microfibrils and other elastic fiber-associated proteins known to regulate elastogenesis do not mediate MAGP-2-induced elastic fiber assembly. Moreover, mutation analysis showed that MAGP-2 does not stimulate elastic fiber assembly through its RGD motif, suggesting that integrin receptor binding does not mediate MAGP-2-induced elastic fiber assembly. Because MAGP-2 interacts with Jagged-1 that controls cell-matrix interaction and cell motility, two key factors in elastic fiber macroassembly, microfibril-associated MAGP-2 may stimulate elastic fiber macroassembly by targeting the release of elastin globules from the cell membrane onto developing elastic fibers."xsd:string |
| http://purl.uniprot.org/citations/17099216 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m609692200"xsd:string |
| http://purl.uniprot.org/citations/17099216 | http://purl.uniprot.org/core/author | "Lafyatis R."xsd:string |
| http://purl.uniprot.org/citations/17099216 | http://purl.uniprot.org/core/author | "Mecham R.P."xsd:string |
| http://purl.uniprot.org/citations/17099216 | http://purl.uniprot.org/core/author | "Bayle J."xsd:string |
| http://purl.uniprot.org/citations/17099216 | http://purl.uniprot.org/core/author | "Lemaire R."xsd:string |
| http://purl.uniprot.org/citations/17099216 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17099216 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/17099216 | http://purl.uniprot.org/core/pages | "800-808"xsd:string |
| http://purl.uniprot.org/citations/17099216 | http://purl.uniprot.org/core/title | "Microfibril-associated MAGP-2 stimulates elastic fiber assembly."xsd:string |
| http://purl.uniprot.org/citations/17099216 | http://purl.uniprot.org/core/volume | "282"xsd:string |
| http://purl.uniprot.org/citations/17099216 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17099216 |
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