| http://purl.uniprot.org/citations/17142383 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17142383 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17142383 | http://www.w3.org/2000/01/rdf-schema#comment | "Geobacillus stearothermophilus T-6 utilizes an extensive and highly regulated hemicellulolytic system. The genes comprising the xylanolytic system are clustered in a 39.7-kb chromosomal segment. This segment contains a 6-kb transcriptional unit (xynDCEFG) coding for a potential two-component system (xynDC) and an ATP-binding cassette (ABC) transport system (xynEFG). The xynD promoter region contains a 16-bp inverted repeat resembling the operator site for the xylose repressor, XylR. XylR was found to bind specifically to this sequence, and binding was efficiently prevented in vitro in the presence of xylose. The ABC transport system was shown to comprise an operon of three genes (xynEFG) that is transcribed from its own promoter. The nonphosphorylated fused response regulator, His6-XynC, bound to a 220-bp fragment corresponding to the xynE operator. DNase I footprinting analysis showed four protected zones that cover the -53 and the +34 regions and revealed direct repeat sequences of a GAAA-like motif. In vitro transcriptional assays and quantitative reverse transcription-PCR demonstrated that xynE transcription is activated 140-fold in the presence of 1.5 microM XynC. The His6-tagged sugar-binding lipoprotein (XynE) of the ABC transporter interacted with different xylosaccharides, as demonstrated by isothermal titration calorimetry. The change in the heat capacity of binding (DeltaCp) for XynE with xylotriose suggests a stacking interaction in the binding site that can be provided by a single Trp residue and a sugar moiety. Taken together, our data show that XynEFG constitutes an ABC transport system for xylo-oligosaccharides and that its transcription is negatively regulated by XylR and activated by the response regulator XynC, which is part of a two-component sensing system."xsd:string |
| http://purl.uniprot.org/citations/17142383 | http://purl.org/dc/terms/identifier | "doi:10.1128/aem.02367-06"xsd:string |
| http://purl.uniprot.org/citations/17142383 | http://purl.org/dc/terms/identifier | "doi:10.1128/aem.02367-06"xsd:string |
| http://purl.uniprot.org/citations/17142383 | http://purl.org/dc/terms/identifier | "doi:10.1128/AEM.02367-06"xsd:string |
| http://purl.uniprot.org/citations/17142383 | http://purl.uniprot.org/core/author | "Sonenshein A.L."xsd:string |
| http://purl.uniprot.org/citations/17142383 | http://purl.uniprot.org/core/author | "Sonenshein A.L."xsd:string |
| http://purl.uniprot.org/citations/17142383 | http://purl.uniprot.org/core/author | "Shoham Y."xsd:string |
| http://purl.uniprot.org/citations/17142383 | http://purl.uniprot.org/core/author | "Shoham Y."xsd:string |
| http://purl.uniprot.org/citations/17142383 | http://purl.uniprot.org/core/author | "Shulami S."xsd:string |
| http://purl.uniprot.org/citations/17142383 | http://purl.uniprot.org/core/author | "Shulami S."xsd:string |
| http://purl.uniprot.org/citations/17142383 | http://purl.uniprot.org/core/author | "Feld G."xsd:string |
| http://purl.uniprot.org/citations/17142383 | http://purl.uniprot.org/core/author | "Feld G."xsd:string |
| http://purl.uniprot.org/citations/17142383 | http://purl.uniprot.org/core/author | "Langut Y."xsd:string |
| http://purl.uniprot.org/citations/17142383 | http://purl.uniprot.org/core/author | "Langut Y."xsd:string |
| http://purl.uniprot.org/citations/17142383 | http://purl.uniprot.org/core/author | "Zaide G."xsd:string |
| http://purl.uniprot.org/citations/17142383 | http://purl.uniprot.org/core/author | "Zaide G."xsd:string |
| http://purl.uniprot.org/citations/17142383 | http://purl.uniprot.org/core/author | "Zolotnitsky G."xsd:string |
| http://purl.uniprot.org/citations/17142383 | http://purl.uniprot.org/core/author | "Zolotnitsky G."xsd:string |
| http://purl.uniprot.org/citations/17142383 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17142383 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17142383 | http://purl.uniprot.org/core/name | "Appl. Environ. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/17142383 | http://purl.uniprot.org/core/name | "Appl. Environ. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/17142383 | http://purl.uniprot.org/core/pages | "874-884"xsd:string |