| http://purl.uniprot.org/citations/1714895 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1714895 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1714895 | http://www.w3.org/2000/01/rdf-schema#comment | "The ubiquitin (Ub)-conjugating enzyme E2(25K) catalyzes the synthesis of multi-Ub chains in which successive Ub units are linked by an isopeptide bond involving the epsilon-amino group of Lys-48 of Ubn, and the COOH-terminal Gly residue of Ubn+1 (Chen, Z., and Pickart, C. M. (1990) J. Biol. Chem., 265, 21835-21842). We now describe the polymerase chain reaction (PCR)-based cloning of an E2(25K)-encoding cDNA from a bovine thymus library, using degenerate oligonucleotide primers based on the sequences of two E2(25K) peptides. The cDNA encodes a 200-residue protein whose sequence bears similarities of 66 and 59%, respectively, to the sequences of the Ub-conjugating enzymes encoded by the UBC1 and UBC4/UBC5 genes of the yeast Saccharomyces cerevisiae. These three yeast E2s play key roles in Ub-dependent proteolysis (Seufert, W., McGrath, J. P., and Jentsch, S. (1990) EMBO J. 9, 4535-4541). Comparison of the amino acid sequence of E2(25K) with other known E2 sequences strongly suggests that Cys-92, one of two E2(25K) Cys residues, forms the Ub thiol ester adduct that is an intermediate in E2-catalyzed multiubiquitination. The E2(25K)-encoding cDNA was overexpressed in Escherichia coli, and the recombinant E2(25K) protein was purified to electrophoretic homogeneity; enzymatic assays showed that its multiubiquitinating activity was quantitatively identical with that of the native protein. The availability of a cloned cDNA will allow us to assess the physiological role of E2(25K)."xsd:string |
| http://purl.uniprot.org/citations/1714895 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(18)98465-6"xsd:string |
| http://purl.uniprot.org/citations/1714895 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(18)98465-6"xsd:string |
| http://purl.uniprot.org/citations/1714895 | http://purl.uniprot.org/core/author | "Chen Z."xsd:string |
| http://purl.uniprot.org/citations/1714895 | http://purl.uniprot.org/core/author | "Chen Z."xsd:string |
| http://purl.uniprot.org/citations/1714895 | http://purl.uniprot.org/core/author | "Niles E.G."xsd:string |
| http://purl.uniprot.org/citations/1714895 | http://purl.uniprot.org/core/author | "Niles E.G."xsd:string |
| http://purl.uniprot.org/citations/1714895 | http://purl.uniprot.org/core/author | "Pickart C.M."xsd:string |
| http://purl.uniprot.org/citations/1714895 | http://purl.uniprot.org/core/author | "Pickart C.M."xsd:string |
| http://purl.uniprot.org/citations/1714895 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/1714895 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/1714895 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/1714895 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/1714895 | http://purl.uniprot.org/core/pages | "15698-15704"xsd:string |
| http://purl.uniprot.org/citations/1714895 | http://purl.uniprot.org/core/pages | "15698-15704"xsd:string |
| http://purl.uniprot.org/citations/1714895 | http://purl.uniprot.org/core/title | "Isolation of a cDNA encoding a mammalian multiubiquitinating enzyme (E225K) and overexpression of the functional enzyme in Escherichia coli."xsd:string |
| http://purl.uniprot.org/citations/1714895 | http://purl.uniprot.org/core/title | "Isolation of a cDNA encoding a mammalian multiubiquitinating enzyme (E225K) and overexpression of the functional enzyme in Escherichia coli."xsd:string |
| http://purl.uniprot.org/citations/1714895 | http://purl.uniprot.org/core/volume | "266"xsd:string |
| http://purl.uniprot.org/citations/1714895 | http://purl.uniprot.org/core/volume | "266"xsd:string |
| http://purl.uniprot.org/citations/1714895 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1714895 |
| http://purl.uniprot.org/citations/1714895 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1714895 |
| http://purl.uniprot.org/citations/1714895 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/1714895 |
| http://purl.uniprot.org/citations/1714895 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/1714895 |