| http://purl.uniprot.org/citations/17158520 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17158520 | http://www.w3.org/2000/01/rdf-schema#comment | "In response to DNA damage, cell cycle arrest, apoptosis, and DNA repair are mediated by a TP53 pathway that induces p21(WAF1/Cip1). The chemotherapeutic drug cis-diamminedichloroplatinum-II (cisplatin) damages cellular DNA by forming cis-diammineplatinum-N(7)-d[GpG] and cis-diammine-platinum-N(7)-d[ApG] adducts. To investigate the role of p21, skin keratinocytes from p21(WAF1/Cip1) wild-type (+/+), heterozygous (+/-), and null (-/-) mice, cultured in calcium levels designed to maintain a proliferating state, were exposed to 5 microM cisplatin continuously for 0, 8, 24, 48 and 72 h. At all time points the (+/-) cells had the fewest Pt-DNA adducts, and at 24 h mean Pt-DNA adduct levels were 541, 153 and 779 fmol adduct/mug DNA for p21(WAF1/Cip1) (+/+), (+/-) and (-/-) cells, respectively [P < 0.05 for (+/+) versus (+/-) and (-/-) versus (+/-)]. In order to understand underlying events, we examined p21(WAF1/Cip1) messenger RNA (mRNA), cell cycle arrest, and apoptosis in these cells. At 48 h of cisplatin exposure p21(WAF1/Cip1) mRNA expression was 2-fold higher in the (+/+) cells, compared to the (+/-) cells. At 24 h, the % of cells in S-phase in cisplatin-exposed cultures, compared to unexposed cultures, was decreased by 51, 40 and 11% in p21(WAF1/Cip1) (+/+), (+/-) and (-/-) cells, respectively (P = 0.04, ANOVA). At 24, 48 and 72 h the % of cisplatin-exposed (+/+) cells in apoptosis was 9.4-10.5%, while the cisplatin-exposed (+/-) and (-/-) cells had 1.2-3.7% of cells in apoptosis. The data support the interpretation that DNA replication arrest and apoptosis do not completely explain the low levels of Pt-DNA adducts in the (+/-) cells, and suggest that p21(WAF1/Cip1) controls activity resulting in either low Pt-DNA adduct formation or enhanced Pt-DNA adduct removal."xsd:string |
| http://purl.uniprot.org/citations/17158520 | http://purl.org/dc/terms/identifier | "doi:10.1093/mutage/gel050"xsd:string |
| http://purl.uniprot.org/citations/17158520 | http://purl.uniprot.org/core/author | "Weinberg W.C."xsd:string |
| http://purl.uniprot.org/citations/17158520 | http://purl.uniprot.org/core/author | "Leil T.A."xsd:string |
| http://purl.uniprot.org/citations/17158520 | http://purl.uniprot.org/core/author | "Divi R.L."xsd:string |
| http://purl.uniprot.org/citations/17158520 | http://purl.uniprot.org/core/author | "Olivero O.A."xsd:string |
| http://purl.uniprot.org/citations/17158520 | http://purl.uniprot.org/core/author | "Poirier M.C."xsd:string |
| http://purl.uniprot.org/citations/17158520 | http://purl.uniprot.org/core/author | "van Gijssel H.E."xsd:string |
| http://purl.uniprot.org/citations/17158520 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17158520 | http://purl.uniprot.org/core/name | "Mutagenesis"xsd:string |
| http://purl.uniprot.org/citations/17158520 | http://purl.uniprot.org/core/pages | "49-54"xsd:string |
| http://purl.uniprot.org/citations/17158520 | http://purl.uniprot.org/core/title | "Cisplatin-DNA damage in p21WAF1/Cip1 deficient mouse keratinocytes exposed to cisplatin."xsd:string |
| http://purl.uniprot.org/citations/17158520 | http://purl.uniprot.org/core/volume | "22"xsd:string |
| http://purl.uniprot.org/citations/17158520 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17158520 |
| http://purl.uniprot.org/citations/17158520 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17158520 |
| http://purl.uniprot.org/uniprot/#_P39689-mappedCitation-17158520 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17158520 |
| http://purl.uniprot.org/uniprot/#_Q4FK34-mappedCitation-17158520 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17158520 |
| http://purl.uniprot.org/uniprot/#_Q564P6-mappedCitation-17158520 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17158520 |
| http://purl.uniprot.org/uniprot/P39689 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/17158520 |
| http://purl.uniprot.org/uniprot/Q4FK34 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/17158520 |
| http://purl.uniprot.org/uniprot/Q564P6 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/17158520 |