| http://purl.uniprot.org/citations/17158937 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17158937 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17158937 | http://www.w3.org/2000/01/rdf-schema#comment | "Little information is available about the molecular mechanisms responsible for polyene resistance in pathogenic yeasts. A clinical isolate of Candida glabrata with a poor susceptibility to polyenes, as determined by disk diffusion method and confirmed by determination of MIC, was recovered from a patient treated with amphotericin B. Quantitative analysis of sterols revealed a lack of ergosterol and an accumulation of late sterol intermediates, suggesting a defect in the final steps of the ergosterol pathway. Sequencing of CgERG11, CgERG6, CgERG5, and CgERG4 genes revealed exclusively a unique missense mutation in CgERG6 leading to the substitution of a cysteine by a phenylalanine in the corresponding protein. In addition, real-time reverse transcription-PCR demonstrated an overexpression of genes encoding enzymes involved in late steps of the ergosterol pathway. Moreover, this isolate exhibited a pseudohyphal growth whatever the culture medium used, and ultrastructural changes of the cell wall of blastoconidia were seen consisting in a thinner inner layer. Cell wall alterations were also suggested by the higher susceptibility of growing cells to Calcofluor white. Additionally, complementation of this isolate with a wild-type copy of the CgERG6 gene restored susceptibility to polyenes and a classical morphology. Together, these results demonstrated that mutation in the CgERG6 gene may lead to a reduced susceptibility to polyenes and to a pseudohyphal growth due to the subsequent changes in sterol content of the plasma membrane."xsd:string |
| http://purl.uniprot.org/citations/17158937 | http://purl.org/dc/terms/identifier | "doi:10.1128/aac.01510-06"xsd:string |
| http://purl.uniprot.org/citations/17158937 | http://purl.org/dc/terms/identifier | "doi:10.1128/aac.01510-06"xsd:string |
| http://purl.uniprot.org/citations/17158937 | http://purl.org/dc/terms/identifier | "doi:10.1128/AAC.01510-06"xsd:string |
| http://purl.uniprot.org/citations/17158937 | http://purl.uniprot.org/core/author | "Hennequin C."xsd:string |
| http://purl.uniprot.org/citations/17158937 | http://purl.uniprot.org/core/author | "Hennequin C."xsd:string |
| http://purl.uniprot.org/citations/17158937 | http://purl.uniprot.org/core/author | "Bouchara J.P."xsd:string |
| http://purl.uniprot.org/citations/17158937 | http://purl.uniprot.org/core/author | "Bouchara J.P."xsd:string |
| http://purl.uniprot.org/citations/17158937 | http://purl.uniprot.org/core/author | "Chabasse D."xsd:string |
| http://purl.uniprot.org/citations/17158937 | http://purl.uniprot.org/core/author | "Chabasse D."xsd:string |
| http://purl.uniprot.org/citations/17158937 | http://purl.uniprot.org/core/author | "Tronchin G."xsd:string |
| http://purl.uniprot.org/citations/17158937 | http://purl.uniprot.org/core/author | "Tronchin G."xsd:string |
| http://purl.uniprot.org/citations/17158937 | http://purl.uniprot.org/core/author | "Vandeputte P."xsd:string |
| http://purl.uniprot.org/citations/17158937 | http://purl.uniprot.org/core/author | "Vandeputte P."xsd:string |
| http://purl.uniprot.org/citations/17158937 | http://purl.uniprot.org/core/author | "Berges T."xsd:string |
| http://purl.uniprot.org/citations/17158937 | http://purl.uniprot.org/core/author | "Berges T."xsd:string |
| http://purl.uniprot.org/citations/17158937 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17158937 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17158937 | http://purl.uniprot.org/core/name | "Antimicrob. Agents Chemother."xsd:string |
| http://purl.uniprot.org/citations/17158937 | http://purl.uniprot.org/core/name | "Antimicrob. Agents Chemother."xsd:string |
| http://purl.uniprot.org/citations/17158937 | http://purl.uniprot.org/core/pages | "982-990"xsd:string |
| http://purl.uniprot.org/citations/17158937 | http://purl.uniprot.org/core/pages | "982-990"xsd:string |
| http://purl.uniprot.org/citations/17158937 | http://purl.uniprot.org/core/title | "Reduced susceptibility to polyenes associated with a missense mutation in the ERG6 gene in a clinical isolate of Candida glabrata with pseudohyphal growth."xsd:string |