| http://purl.uniprot.org/citations/17182860 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17182860 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17182860 | http://www.w3.org/2000/01/rdf-schema#comment | "Cdc42-associated tyrosine kinase 1 (ACK1) is a specific down-stream effector of Cdc42, a Rho family small G-protein. Previous studies have shown that ACK1 interacts with clathrin heavy chain and is involved in clathrin-coated vesicle endocytosis. Here we report that ACK1 interacted with epidermal growth factor receptor (EGFR) upon EGF stimulation via a region at carboxy terminus that is highly homologous to Gene-33/Mig-6/RALT. The interaction of ACK1 with EGFR was dependent on the kinase activity or tyrosine phosphorylation of EGFR. Immunofluorescent staining using anti-EGFR and GFP-ACK1 indicates that ACK1 was colocalized with EGFR on EEA-1 positive vesicles upon EGF stimulation. Suppression of the expression of ACK1 by ACK-RNAi inhibited ligand-induced degradation of EGFR upon EGF stimulation, suggesting that ACK1 plays an important role in regulation of EGFR degradation in cells. Furthermore, we identified ACK1 as an ubiquitin-binding protein. Through an ubiquitin-association (Uba) domain at the carboxy terminus, ACK1 binds to both poly- and mono-ubiquitin. Overexpression of the Uba domain-deletion mutant of ACK1 blocked the ligand-dependent degradation of EGFR, suggesting that ACK1 regulates EGFR degradation via its Uba domain. Taken together, our studies suggest that ACK1 senses signal of EGF and regulates ligand-induced degradation of EGFR."xsd:string |
| http://purl.uniprot.org/citations/17182860 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.e06-02-0142"xsd:string |
| http://purl.uniprot.org/citations/17182860 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.e06-02-0142"xsd:string |
| http://purl.uniprot.org/citations/17182860 | http://purl.uniprot.org/core/author | "Gu Y."xsd:string |
| http://purl.uniprot.org/citations/17182860 | http://purl.uniprot.org/core/author | "Gu Y."xsd:string |
| http://purl.uniprot.org/citations/17182860 | http://purl.uniprot.org/core/author | "Lin Q."xsd:string |
| http://purl.uniprot.org/citations/17182860 | http://purl.uniprot.org/core/author | "Lin Q."xsd:string |
| http://purl.uniprot.org/citations/17182860 | http://purl.uniprot.org/core/author | "Yang W."xsd:string |
| http://purl.uniprot.org/citations/17182860 | http://purl.uniprot.org/core/author | "Yang W."xsd:string |
| http://purl.uniprot.org/citations/17182860 | http://purl.uniprot.org/core/author | "Childress C."xsd:string |
| http://purl.uniprot.org/citations/17182860 | http://purl.uniprot.org/core/author | "Childress C."xsd:string |
| http://purl.uniprot.org/citations/17182860 | http://purl.uniprot.org/core/author | "Shen F."xsd:string |
| http://purl.uniprot.org/citations/17182860 | http://purl.uniprot.org/core/author | "Shen F."xsd:string |
| http://purl.uniprot.org/citations/17182860 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17182860 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17182860 | http://purl.uniprot.org/core/name | "Mol. Biol. Cell"xsd:string |
| http://purl.uniprot.org/citations/17182860 | http://purl.uniprot.org/core/name | "Mol. Biol. Cell"xsd:string |
| http://purl.uniprot.org/citations/17182860 | http://purl.uniprot.org/core/pages | "732-742"xsd:string |
| http://purl.uniprot.org/citations/17182860 | http://purl.uniprot.org/core/pages | "732-742"xsd:string |
| http://purl.uniprot.org/citations/17182860 | http://purl.uniprot.org/core/title | "Activated Cdc42-associated kinase 1 is a component of EGF receptor signaling complex and regulates EGF receptor degradation."xsd:string |
| http://purl.uniprot.org/citations/17182860 | http://purl.uniprot.org/core/title | "Activated Cdc42-associated kinase 1 is a component of EGF receptor signaling complex and regulates EGF receptor degradation."xsd:string |
| http://purl.uniprot.org/citations/17182860 | http://purl.uniprot.org/core/volume | "18"xsd:string |
| http://purl.uniprot.org/citations/17182860 | http://purl.uniprot.org/core/volume | "18"xsd:string |