| http://purl.uniprot.org/citations/17183061 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17183061 | http://www.w3.org/2000/01/rdf-schema#comment | "n-3 Polyunsaturated fatty acids have been shown to powerfully inhibit the growth of colon cancer cells, mainly acting as pro-apoptotic agents through inhibition of cycloxygenase-2 (COX-2) expression. Since dysregulation of beta-catenin expression is frequently found at early stage of colorectal carcinogenesis, we analyzed whether docosahexaenoic acid (DHA) may modify the expression of beta-catenin in colon cancer cells (SW480 and HCT116) over-expressing this protein, but lacking COX-2. Futhermore, we investigated if alterations in beta-catenin expression may be associated with apoptosis induction. Treatment of cells with increasing concentrations of DHA induced a dose- and time-dependent inhibition of beta-catenin protein expression which, however, was not accompanied by modifications in beta-catenin transcription. Conversely, the proteasomal inhibitors MG132 and lactacystin prevented DHA-induced beta-catenin decrease, suggesting that DHA may regulate the proteasomal degradation of beta-catenin. The reduced levels of beta-catenin were accompanied by decreased translocation of beta-catenin into the nucleus, where it acts as a transcription factor in concert with T-Cell Factor (TCF). DHA, at the same range of concentrations, was also able to induce apoptosis by a caspase-3-dependent mechanism and to cause a dose- and time-dependent decrease of survivin, an apoptosis inhibitor undetectable in normal tissues and expressed in colorectal cancer through TCF-beta-catenin stimulation. Several other proteins regulated by the TCF-beta-catenin pathway and involved in regulation of tumor growth were down-regulated by DHA, including peroxisome proliferator-activated receptor-delta, membrane type 1 (MT1)-matrix metalloproteinase (MMP), MMP-7 and vascular endothelial growth factor. The present study, thus, raises the possibility that DHA may exert pro-apoptotic and antitumoral effects through proteasomal regulation of beta-catenin levels and alterations in the expression of TCF-beta-catenin target genes."xsd:string |
| http://purl.uniprot.org/citations/17183061 | http://purl.org/dc/terms/identifier | "doi:10.1093/carcin/bgl254"xsd:string |
| http://purl.uniprot.org/citations/17183061 | http://purl.uniprot.org/core/author | "Serini S."xsd:string |
| http://purl.uniprot.org/citations/17183061 | http://purl.uniprot.org/core/author | "Ranelletti F.O."xsd:string |
| http://purl.uniprot.org/citations/17183061 | http://purl.uniprot.org/core/author | "Monego G."xsd:string |
| http://purl.uniprot.org/citations/17183061 | http://purl.uniprot.org/core/author | "Toesca A."xsd:string |
| http://purl.uniprot.org/citations/17183061 | http://purl.uniprot.org/core/author | "Boninsegna A."xsd:string |
| http://purl.uniprot.org/citations/17183061 | http://purl.uniprot.org/core/author | "Calviello G."xsd:string |
| http://purl.uniprot.org/citations/17183061 | http://purl.uniprot.org/core/author | "Palozza P."xsd:string |
| http://purl.uniprot.org/citations/17183061 | http://purl.uniprot.org/core/author | "Piccioni E."xsd:string |
| http://purl.uniprot.org/citations/17183061 | http://purl.uniprot.org/core/author | "Resci F."xsd:string |
| http://purl.uniprot.org/citations/17183061 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17183061 | http://purl.uniprot.org/core/name | "Carcinogenesis"xsd:string |
| http://purl.uniprot.org/citations/17183061 | http://purl.uniprot.org/core/pages | "1202-1209"xsd:string |
| http://purl.uniprot.org/citations/17183061 | http://purl.uniprot.org/core/title | "Docosahexaenoic acid induces proteasome-dependent degradation of beta-catenin, down-regulation of survivin and apoptosis in human colorectal cancer cells not expressing COX-2."xsd:string |
| http://purl.uniprot.org/citations/17183061 | http://purl.uniprot.org/core/volume | "28"xsd:string |
| http://purl.uniprot.org/citations/17183061 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17183061 |
| http://purl.uniprot.org/citations/17183061 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17183061 |
| http://purl.uniprot.org/uniprot/#_Q15008-mappedCitation-17183061 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17183061 |
| http://purl.uniprot.org/uniprot/#_A5LHX3-mappedCitation-17183061 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17183061 |
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| http://purl.uniprot.org/uniprot/#_O43242-mappedCitation-17183061 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17183061 |
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| http://purl.uniprot.org/uniprot/#_P0CG47-mappedCitation-17183061 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17183061 |