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http://purl.uniprot.org/citations/17188392http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/17188392http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/17188392http://www.w3.org/2000/01/rdf-schema#comment"

Background/aims

Hepatitis C virus genotype-3a (HCV-3a) is directly linked to steatosis development. We studied the effects of HCV-3a core protein on the promoter activity of fatty acid synthase (FAS), a major enzyme involved in de novo lipid synthesis.

Methods and results

HCV-3a and -1b core genes were cloned and expressed. Using a FAS promoter-luciferase reporter, we demonstrated that both HCV-3a and -1b core proteins up-regulated the FAS promoter. However, HCV-3a core protein expression induced significantly higher FAS promoter activity than HCV-1b core. We further showed that FAS up-regulation by HCV core was dependent on transcription factor sterol response element binding protein-1. Mutational analysis showed that processing of HCV core protein of different genotypes was differentially involved in FAS promoter up-regulation. Although lipid droplet localization of HCV core protein was not important for FAS up-regulation, a specific amino acid residue (Phe(164)) within the FATG lipid droplet localization sequence of HCV-3a core protein played a major role in the stronger FAS activation by HCV-3a core.

Conclusions

The stronger effect of HCV-3a core protein on FAS activation in comparison to HCV-1b core could contribute to the higher prevalence and severity of steatosis in HCV-3a infections."xsd:string
http://purl.uniprot.org/citations/17188392http://purl.org/dc/terms/identifier"doi:10.1016/j.jhep.2006.10.019"xsd:string
http://purl.uniprot.org/citations/17188392http://purl.org/dc/terms/identifier"doi:10.1016/j.jhep.2006.10.019"xsd:string
http://purl.uniprot.org/citations/17188392http://purl.uniprot.org/core/author"Babiuk L.A."xsd:string
http://purl.uniprot.org/citations/17188392http://purl.uniprot.org/core/author"Babiuk L.A."xsd:string
http://purl.uniprot.org/citations/17188392http://purl.uniprot.org/core/author"Liu Q."xsd:string
http://purl.uniprot.org/citations/17188392http://purl.uniprot.org/core/author"Liu Q."xsd:string
http://purl.uniprot.org/citations/17188392http://purl.uniprot.org/core/author"Jackel-Cram C."xsd:string
http://purl.uniprot.org/citations/17188392http://purl.uniprot.org/core/author"Jackel-Cram C."xsd:string
http://purl.uniprot.org/citations/17188392http://purl.uniprot.org/core/date"2007"xsd:gYear
http://purl.uniprot.org/citations/17188392http://purl.uniprot.org/core/date"2007"xsd:gYear
http://purl.uniprot.org/citations/17188392http://purl.uniprot.org/core/name"J. Hepatol."xsd:string
http://purl.uniprot.org/citations/17188392http://purl.uniprot.org/core/name"J. Hepatol."xsd:string
http://purl.uniprot.org/citations/17188392http://purl.uniprot.org/core/pages"999-1008"xsd:string
http://purl.uniprot.org/citations/17188392http://purl.uniprot.org/core/pages"999-1008"xsd:string
http://purl.uniprot.org/citations/17188392http://purl.uniprot.org/core/title"Up-regulation of fatty acid synthase promoter by hepatitis C virus core protein: genotype-3a core has a stronger effect than genotype-1b core."xsd:string
http://purl.uniprot.org/citations/17188392http://purl.uniprot.org/core/title"Up-regulation of fatty acid synthase promoter by hepatitis C virus core protein: genotype-3a core has a stronger effect than genotype-1b core."xsd:string
http://purl.uniprot.org/citations/17188392http://purl.uniprot.org/core/volume"46"xsd:string
http://purl.uniprot.org/citations/17188392http://purl.uniprot.org/core/volume"46"xsd:string
http://purl.uniprot.org/citations/17188392http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/17188392
http://purl.uniprot.org/citations/17188392http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/17188392
http://purl.uniprot.org/citations/17188392http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/17188392
http://purl.uniprot.org/citations/17188392http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/17188392
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