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http://purl.uniprot.org/citations/17192717http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/17192717http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/17192717http://www.w3.org/2000/01/rdf-schema#comment"

Background

Cry j 2 is one of the major pollen allergens of Cryptomeria japonica. The polymorphism of Cry j 2 isoforms and the conservation of the structure of Cry j 2 in coniferous species remain to be analyzed.

Methods

A cDNA library derived from the pollen of C. japonica was screened using a fragment of Cry j 2 cDNA. Restriction fragment length polymorphism analysis was performed to examine the diversity of Cry j 2 genes. The promoters of Cry j 2 genes were isolated with a commercially available cloning kit. Clonal variations in the expression of Cry j 2 in pollen were examined by RNA gel blot analysis, and the conservation of the structure of the Cry j 2 gene in coniferous species was evaluated by DNA gel blot analysis.

Results

We isolated three cDNA clones encoding novel isoforms of Cry j 2. We also sequenced a total of 16 promoter regions from 10 specimens. The sequences of promoter regions of Cry j 2 genes were highly divergent. The amount of Cry j 2 mRNA also varied considerably. The Cry j 2 gene was found to be conserved among species belonging to Taxodiaceae and Cupressaceae but to vary between Taxodiaceae and Pinaceae.

Conclusions

The coding and promoter regions of Cry j 2 genes contain large numbers of polymorphisms. Our analysis revealed large variations in the expression of Cry j 2 at the transcriptional level, and we suggest that conserved homologs of Cry j 2 confer cross-allergenicity among Taxodiaceae and Cupressaceae."xsd:string
http://purl.uniprot.org/citations/17192717http://purl.org/dc/terms/identifier"doi:10.1159/000098225"xsd:string
http://purl.uniprot.org/citations/17192717http://purl.org/dc/terms/identifier"doi:10.1159/000098225"xsd:string
http://purl.uniprot.org/citations/17192717http://purl.uniprot.org/core/author"Mukai Y."xsd:string
http://purl.uniprot.org/citations/17192717http://purl.uniprot.org/core/author"Mukai Y."xsd:string
http://purl.uniprot.org/citations/17192717http://purl.uniprot.org/core/author"Futamura N."xsd:string
http://purl.uniprot.org/citations/17192717http://purl.uniprot.org/core/author"Futamura N."xsd:string
http://purl.uniprot.org/citations/17192717http://purl.uniprot.org/core/author"Shinohara K."xsd:string
http://purl.uniprot.org/citations/17192717http://purl.uniprot.org/core/author"Shinohara K."xsd:string
http://purl.uniprot.org/citations/17192717http://purl.uniprot.org/core/author"Kusunoki Y."xsd:string
http://purl.uniprot.org/citations/17192717http://purl.uniprot.org/core/author"Kusunoki Y."xsd:string
http://purl.uniprot.org/citations/17192717http://purl.uniprot.org/core/date"2007"xsd:gYear
http://purl.uniprot.org/citations/17192717http://purl.uniprot.org/core/date"2007"xsd:gYear
http://purl.uniprot.org/citations/17192717http://purl.uniprot.org/core/name"Int. Arch. Allergy Immunol."xsd:string
http://purl.uniprot.org/citations/17192717http://purl.uniprot.org/core/name"Int Arch Allergy Immunol"xsd:string
http://purl.uniprot.org/citations/17192717http://purl.uniprot.org/core/pages"59-68"xsd:string
http://purl.uniprot.org/citations/17192717http://purl.uniprot.org/core/pages"59-68"xsd:string
http://purl.uniprot.org/citations/17192717http://purl.uniprot.org/core/title"Characterization of genes for a pollen allergen, Cry j 2, of Cryptomeria japonica."xsd:string
http://purl.uniprot.org/citations/17192717http://purl.uniprot.org/core/title"Characterization of genes for a pollen allergen, Cry j 2, of Cryptomeria japonica."xsd:string
http://purl.uniprot.org/citations/17192717http://purl.uniprot.org/core/volume"143"xsd:string
http://purl.uniprot.org/citations/17192717http://purl.uniprot.org/core/volume"143"xsd:string
http://purl.uniprot.org/citations/17192717http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/17192717
http://purl.uniprot.org/citations/17192717http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/17192717
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