| http://purl.uniprot.org/citations/17234882 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17234882 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17234882 | http://www.w3.org/2000/01/rdf-schema#comment | "Spt6 promotes transcription elongation at many genes and functions as a histone H3 chaperone to alter chromatin structure during transcription. We show here that mammalian Spt6 binds Ser2-phosphorylated (Ser2P) RNA polymerase II (RNAPII) through a primitive SH2 domain, which recognizes phosphoserine rather than phosphotyrosine residues. Surprisingly, a point mutation in the Spt6 SH2 domain (R1358K) blocked binding to RNAPIIo without affecting transcription elongation rates in vitro. However, HIV-1 and c-myc RNAs formed in cells expressing the mutant Spt6 protein were longer than normal and contained splicing defects. Ectopic expression of the wild-type, but not mutant, Spt6 SH2 domain, caused bulk poly(A)+ RNAs to be retained in the nucleus, further suggesting a widespread role for Spt6 in mRNA processing or assembly of export-competent mRNP particles. We cloned the human Spt6-interacting protein, hIws1 (interacts with Spt6), and found that it associates with the nuclear RNA export factor, REF1/Aly. Depletion of endogenous hIws1 resulted in mRNA processing defects, lower levels of REF1/Aly at the c-myc gene, and nuclear retention of bulk HeLa poly(A)+ RNAs in vivo. Thus binding of Spt6 to Ser2-P RNAPII provides a cotranscriptional mechanism to recruit Iws1, REF1/Aly, and associated mRNA processing, surveillance, and export factors to responsive genes."xsd:string |
| http://purl.uniprot.org/citations/17234882 | http://purl.org/dc/terms/identifier | "doi:10.1101/gad.1503107"xsd:string |
| http://purl.uniprot.org/citations/17234882 | http://purl.org/dc/terms/identifier | "doi:10.1101/gad.1503107"xsd:string |
| http://purl.uniprot.org/citations/17234882 | http://purl.uniprot.org/core/author | "Jones K.A."xsd:string |
| http://purl.uniprot.org/citations/17234882 | http://purl.uniprot.org/core/author | "Jones K.A."xsd:string |
| http://purl.uniprot.org/citations/17234882 | http://purl.uniprot.org/core/author | "Evans R.M."xsd:string |
| http://purl.uniprot.org/citations/17234882 | http://purl.uniprot.org/core/author | "Evans R.M."xsd:string |
| http://purl.uniprot.org/citations/17234882 | http://purl.uniprot.org/core/author | "Cho H."xsd:string |
| http://purl.uniprot.org/citations/17234882 | http://purl.uniprot.org/core/author | "Cho H."xsd:string |
| http://purl.uniprot.org/citations/17234882 | http://purl.uniprot.org/core/author | "Yoh S.M."xsd:string |
| http://purl.uniprot.org/citations/17234882 | http://purl.uniprot.org/core/author | "Yoh S.M."xsd:string |
| http://purl.uniprot.org/citations/17234882 | http://purl.uniprot.org/core/author | "Pickle L."xsd:string |
| http://purl.uniprot.org/citations/17234882 | http://purl.uniprot.org/core/author | "Pickle L."xsd:string |
| http://purl.uniprot.org/citations/17234882 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17234882 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17234882 | http://purl.uniprot.org/core/name | "Genes Dev."xsd:string |
| http://purl.uniprot.org/citations/17234882 | http://purl.uniprot.org/core/name | "Genes Dev."xsd:string |
| http://purl.uniprot.org/citations/17234882 | http://purl.uniprot.org/core/pages | "160-174"xsd:string |
| http://purl.uniprot.org/citations/17234882 | http://purl.uniprot.org/core/pages | "160-174"xsd:string |
| http://purl.uniprot.org/citations/17234882 | http://purl.uniprot.org/core/title | "The Spt6 SH2 domain binds Ser2-P RNAPII to direct Iws1-dependent mRNA splicing and export."xsd:string |
| http://purl.uniprot.org/citations/17234882 | http://purl.uniprot.org/core/title | "The Spt6 SH2 domain binds Ser2-P RNAPII to direct Iws1-dependent mRNA splicing and export."xsd:string |
| http://purl.uniprot.org/citations/17234882 | http://purl.uniprot.org/core/volume | "21"xsd:string |
| http://purl.uniprot.org/citations/17234882 | http://purl.uniprot.org/core/volume | "21"xsd:string |