| http://purl.uniprot.org/citations/17244820 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17244820 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17244820 | http://www.w3.org/2000/01/rdf-schema#comment | "Protein kinase A anchoring proteins or AKAPs regulate the activity of many ion channels. Protein kinase A (PKA) is a well-recognized target of AKAPs, with other kinases now emerging as additional targets. We examined the roles of epithelial-expressed AKAPs in regulating the epithelial Na+ channel (ENaC). Experiments used heterologous expression with AKAP15, AKAP-KL, and AKAP79 in Xenopus oocytes. Experiments were carried out under high and low Na+ conditions, as Na+ loading is known to affect the baseline activity of ENaC in a PKC-dependent mechanism. ENaC activity was unaffected by AKAP79 and AKAP-KL expression. However, oocytes coexpressing AKAP15 exhibited an 80% and 91% reduction in the amiloride-sensitive, whole-cell conductance in high and low Na+ conditions, respectively. The reduced channel activity was unaffected by PKA activation or inhibition, indicating a PKA-independent mechanism. Expression with a membrane-targeting domain, mutant form of AKAP15 (AKAP15m) prevented the decrease of ENaC activity, but only under low Na+ conditions. In high sodium conditions, coexpression with AKAP15m led to an increase of ENaC activity to levels similar to those observed under low Na+. These results indicate that membrane-associated AKAP15 reduces ENaC activity whereas the cytoplasmically associated one may participate in the channel's feedback inhibition by intracellular Na+, a process known to involve PKC. This hypothesis was further confirmed in coexpression experiments, which demonstrated functional and physical interaction between AKAP15 and PKCalpha. We propose that AKAP15 regulates ENaC via a novel PKA-independent pathway."xsd:string |
| http://purl.uniprot.org/citations/17244820 | http://purl.org/dc/terms/identifier | "doi:10.1096/fj.06-6046com"xsd:string |
| http://purl.uniprot.org/citations/17244820 | http://purl.org/dc/terms/identifier | "doi:10.1096/fj.06-6046com"xsd:string |
| http://purl.uniprot.org/citations/17244820 | http://purl.uniprot.org/core/author | "Li J."xsd:string |
| http://purl.uniprot.org/citations/17244820 | http://purl.uniprot.org/core/author | "Li J."xsd:string |
| http://purl.uniprot.org/citations/17244820 | http://purl.uniprot.org/core/author | "Bengrine A."xsd:string |
| http://purl.uniprot.org/citations/17244820 | http://purl.uniprot.org/core/author | "Bengrine A."xsd:string |
| http://purl.uniprot.org/citations/17244820 | http://purl.uniprot.org/core/author | "Awayda M.S."xsd:string |
| http://purl.uniprot.org/citations/17244820 | http://purl.uniprot.org/core/author | "Awayda M.S."xsd:string |
| http://purl.uniprot.org/citations/17244820 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17244820 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17244820 | http://purl.uniprot.org/core/name | "FASEB J."xsd:string |
| http://purl.uniprot.org/citations/17244820 | http://purl.uniprot.org/core/name | "FASEB J."xsd:string |
| http://purl.uniprot.org/citations/17244820 | http://purl.uniprot.org/core/pages | "1189-1201"xsd:string |
| http://purl.uniprot.org/citations/17244820 | http://purl.uniprot.org/core/pages | "1189-1201"xsd:string |
| http://purl.uniprot.org/citations/17244820 | http://purl.uniprot.org/core/title | "The A-kinase anchoring protein 15 regulates feedback inhibition of the epithelial Na+ channel."xsd:string |
| http://purl.uniprot.org/citations/17244820 | http://purl.uniprot.org/core/title | "The A-kinase anchoring protein 15 regulates feedback inhibition of the epithelial Na+ channel."xsd:string |
| http://purl.uniprot.org/citations/17244820 | http://purl.uniprot.org/core/volume | "21"xsd:string |
| http://purl.uniprot.org/citations/17244820 | http://purl.uniprot.org/core/volume | "21"xsd:string |
| http://purl.uniprot.org/citations/17244820 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17244820 |
| http://purl.uniprot.org/citations/17244820 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17244820 |
| http://purl.uniprot.org/citations/17244820 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17244820 |
| http://purl.uniprot.org/citations/17244820 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17244820 |